Detail Information for IndEnz0010001063
IED ID IndEnz0010001063
Enzyme Type ID esterase001063
Protein Name Lysophospholipase nte1
EC 3.1.1.5
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene Name nte1 ACLA_055880
Organism Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus clavatus Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)
Enzyme Sequence MADGNLLGSSTSLTALLPTPSSASLSASLSSSSLPVSPFLAPAPTTAITASIASLSAQPPPPLPATPATMAGWIGWVFSFFFQFIPSVLYSVITFTTITLPTWLFTLFSMSLTFTMNFTTLLLILLAVVSTLGWFVRYRFLNMYSRLPPEPQRKEPQIDLFPDVQGGDSKPGLANYLDEFLSAIKVFGYLERPVFHELTRTMQTRKLIAGETLMLEEEKGFCLVVDGLVQIFVKSTRDGKSGSDDELHHLGAESSDEEHHIDGKQGYQLLTEVKNGASMSSLFSILSLFTEDIQVWDSQSSTSSSSSIAMRAARVPDSTPNSPRGGMDSPTPIFRDVPDPVSLVNENGDLPLVPPLHLEESPIPPTNHAHDRRQHDHRKHHGRKHRKSVHPDIVARAMVDTTIAIIPASAFRRLTRVYPRATAHIVQVILTRLQRVTFATAHSYLGLSNEVLGIEKQMTKFTTYDLPNNMRGTALDRLKDKFIKERDRLGTEEVTKGIALHNPSAGRRRRSSSFMRKDAVLHAKMMSPKRAATVITSDNSYDHDSAGVSPGDLLSTIQQSRFGPRYEQPTPRLRSPLAEKENSHFRLPAMQARNAFHRKESLDEDALFRECILDCIMKAIGLTSSTGEVLRKSSHSGEASPKLLSYDSRRQKAVFSNNAFGFIDPYEGSGDGETESMMSMSVTSAGGTSPVTSLREELRNDIEIVYFPQGSVLVEQGERHPGLYYVIDGFLDVGMPVVDKGEDLVGVSKPATAREPFPTLKRTTTASSIKPSATAANDPRRRKQSRKSLYLIKPGGIQGYVGAVASYRSYTDVVAKTDVYVGFLPRASLERIAERYPIALLTLAKRLTSLLPRLLLHIDFALEWVQVNAGQVIYHQGDESDAIYLVLNGRLRSVLESADNKLTVIGEYGQGESVGELEVMTESTRPATLHAIRDTELAKFPRSLFNSLAQEHPGITIQVSKLIAQRMRDLVERPVTEKGAERSSAGGVQTATSTLNLRTVGILPVTAGVPVVEFGNRLLHALHQIGVVNGVTSLNQSAILNHLGRHAFSKMGKLKLAQYLADLEEKYGMVLYIADTNVNSPWTQTCITQADCILLVGLAESSPSIGEYERFLLGMKTTARKELVLLHSERYCPPGLTRRWLKNRVWINGGHHHIQMAFRLTAEPSHPETKRFGTVLKQRVQVLQAEIQKYTSRRIRQTPLYSAQTPFKGDFHRLARRLCGRAVGLVLGGGGARGIAHVGVIKALEEAGIPVDIIGGTSIGSFIGALYARDADVVPMYGRAKKFAGRMGSMWRFALDLTYPTISYTTGHEFNRGIFKTFGDSQIEDFWLEFYCNTTNISKSRQEYHSSGYVWRYVRASMSLAGLIPPICDEGSMLLDGGYIDNLTVDHMKGLGADVIFAVDVGSIDDNTPQGYGDSLSGFWVAFNRWNPFSSCPNPPTLSEIQARLAYVSSIDNLERAKITPGCLYMRPPIDAYGTLEFGKFDEIYQVGYKFGKQFLEKLKNEGSLPLPEETEEEKKLLRTMAPRRASI
Enzyme Length 1528
Uniprot Accession Number A1C9L6
Absorption
Active Site ACT_SITE 1258; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1376; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161
Activity Regulation ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5;
DNA Binding
EC Number 3.1.1.5
Enzyme Function FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 686..805; /note=cNMP 1; NP_BIND 846..966; /note=cNMP 2
Features Active site (2); Chain (1); Compositional bias (2); Domain (1); Erroneous initiation (1); Motif (3); Nucleotide binding (2); Region (5); Topological domain (3); Transmembrane (2)
Keywords Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 1229..1234; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1256..1260; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1376..1378; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161
Gene Encoded By
Mass 168,937
Kinetics
Metal Binding
Rhea ID RHEA:15177
Cross Reference Brenda