| IED ID | IndEnz0010001067 |
| Enzyme Type ID | esterase001067 |
| Protein Name |
Palmitoleoyl-protein carboxylesterase NOTUM EC 3.1.1.98 hNOTUM |
| Gene Name | NOTUM OK/SW-CL.30 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MGRGVRVLLLLSLLHCAGGSEGRKTWRRRGQQPPPPPRTEAAPAAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESRGSRRWLLFLEGGWYCFNRENCDSRYDTMRRLMSSRDWPRTRTGTGILSSQPEENPYWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQEVVRELLGRGLSGAKVLLLAGSSAGGTGVLLNVDRVAEQLEKLGYPAIQVRGLADSGWFLDNKQYRHTDCVDTITCAPTEAIRRGIRYWNGVVPERCRRQFQEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGLRLYIQNLGRELRHTLKDVPASFAPACLSHEIIIRSHWTDVQVKGTSLPRALHCWDRSLHDSHKASKTPLKGCPVHLVDSCPWPHCNPSCPTVRDQFTGQEMNVAQFLMHMGFDMQTVAQPQGLEPSELLGMLSNGS |
| Enzyme Length | 496 |
| Uniprot Accession Number | Q6P988 |
| Absorption | |
| Active Site | ACT_SITE 232; /note=Charge relay system; /evidence=ECO:0000269|PubMed:25731175; ACT_SITE 340; /note=Charge relay system; /evidence=ECO:0000269|PubMed:25731175; ACT_SITE 389; /note=Charge relay system; /evidence=ECO:0000269|PubMed:25731175 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[Wnt protein]-O-(9Z)-hexadecenoyl-L-serine + H2O = (9Z)-hexadecenoate + [Wnt protein]-L-serine + H(+); Xref=Rhea:RHEA:45340, Rhea:RHEA-COMP:11170, Rhea:RHEA-COMP:11171, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:32372, ChEBI:CHEBI:85189; EC=3.1.1.98; Evidence={ECO:0000269|PubMed:25731175}; |
| DNA Binding | |
| EC Number | 3.1.1.98 |
| Enzyme Function | FUNCTION: Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed:25731175). {ECO:0000269|PubMed:25731175}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (19); Chain (1); Erroneous initiation (3); Glycosylation (1); Helix (15); Modified residue (1); Mutagenesis (1); Region (1); Sequence conflict (2); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Glycoprotein;Hydrolase;Phosphoprotein;Reference proteome;Secreted;Serine esterase;Signal;Wnt signaling pathway |
| Interact With | Q8N720 |
| Induction | INDUCTION: Up-regulated in hepatocellular carcinoma (HCC) with high intracellular beta-catenin. {ECO:0000269|PubMed:18429952}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VUX3}. |
| Modified Residue | MOD_RES 81; /note=Phosphoserine; by FAM20C; /evidence=ECO:0000269|PubMed:26091039 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (100) |
| Cross Reference PDB | 4UYU; 4UYW; 4UYZ; 4UZ1; 4UZ5; 4UZ6; 4UZ7; 4UZ9; 4UZA; 4UZL; 4UZQ; 4WBH; 6R8P; 6R8Q; 6R8R; 6T2H; 6T2K; 6TR5; 6TR6; 6TR7; 6TUZ; 6TV4; 6YSK; 6YUW; 6YUY; 6YV0; 6YV2; 6YV4; 6YXI; 6ZUV; 6ZVL; 6ZYF; 7ARG; 7B2V; 7B2Y; 7B2Z; 7B37; 7B3F; 7B3G; 7B3H; 7B3I; 7B3P; 7B3X; 7B45; 7B4X; 7B50; 7B7W; 7B7X; 7B7Y; 7B84; 7B86; 7B87; 7B89; 7B8A; 7B8C; 7B8D; 7B8F; 7B8G; 7B8J; 7B8K; 7B8L; 7B8M; 7B8N; 7B8O; 7B8U; 7B8X; 7B8Y; 7B8Z; 7B98; 7B99; 7B9D; 7B9I; 7B9N; 7B9U; 7BA1; 7BAC; 7BAP; 7BC8; 7BC9; 7BCC; 7BCD; 7BCF; 7BCH; 7BCI; 7BCK; 7BCL; 7BD2; 7BD3; 7BD4; 7BD5; 7BD6; 7BD8; 7BD9; 7BDA; 7BDB; 7BDC; 7BDD; 7BDF; 7BDG; 7BDH; |
| Mapped Pubmed ID | 15647319; 15963974; 17609110; 17967162; 20412775; 26517809; 30343282; 31292548; 31307226; 31534655; 31862412; 31876313; 32787107; 33033363; 33124429; 33647468; 34079124; 34292747; 34402722; |
| Motif | |
| Gene Encoded By | |
| Mass | 55,699 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:45340 |
| Cross Reference Brenda | 3.1.1.98; |