| IED ID | IndEnz0010001081 |
| Enzyme Type ID | esterase001081 |
| Protein Name |
Transcription factor mokH Monacolin K biosynthesis protein H |
| Gene Name | mokH |
| Organism | Monascus pilosus (Red mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Monascus Monascus pilosus (Red mold) |
| Enzyme Sequence | MALSPVQDPPSHTDKTMPRRAFRRSCDRCHAQKIKCIGSEGAVARASCQRCQQAGLRCVYSERCPKRKLPKPNPAESSPASSTAGLHTSSSDSSPPVPSDGLPLDLPGPDSSGVSLQFLDPSADCDWPWSSIGVDETVVNNCLDLSHGHGHGDLSCQLELPMPDLPSPFEFSAEKSPSPSVSGSIAGAVSAQRELFDGLSTVSQELEAILLAVAVEWPKQEIWTYPIGTFFNASRRLLVYLQQQSNTRSDQGMLNECLRTKNLFMAVHCYMLIVKIFTSLSELLLSQIRHSQAGQLTPLEGHQFEPPPSSSRDRSSVDTMPIFNPNLHIGGLFSYLNPFMHALSSACTTLRVGVQLLRENESALGIPPAQGVAASVSMGKEEWADGEDVASAVTTADEDLRQPASRILSMVWSDEVGDQKAKSADAAGPRSRTLAVLRRCNREIFSLARQHNLAS |
| Enzyme Length | 455 |
| Uniprot Accession Number | Q3S2U4 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | DNA_BIND 26..58; /note=Zn(2)-C6 fungal-type; /evidence=ECO:0000255|PROSITE-ProRule:PRU00227 |
| EC Number | |
| Enzyme Function | FUNCTION: Transcription factor that regulates the gene cluster that mediates the biosynthesis of monakolin K, also known as lovastatin, and which acts as a potent competitive inhibitor of HMG-CoA reductase (PubMed:18578535, PubMed:19693441, PubMed:19968298). Monakolin K biosynthesis is performed in two stages (PubMed:19693441). The first stage is catalyzed by the nonaketide synthase mokA, which belongs to type I polyketide synthases and catalyzes the iterative nine-step formation of the polyketide (PubMed:18578535, PubMed:19693441). This PKS stage is completed by the action of dehydrogenase mokE, which catalyzes the NADPH-dependent reduction of the unsaturated tetra-, penta- and heptaketide intermediates that arise during the mokA-mediated biosynthesis of the nonaketide chain and leads to dihydromonacolin L (PubMed:19693441). Covalently bound dihydromonacolin L is released from mokA by the mokD esterase (By similarity). Conversion of dihydromonacolin L into monacolin L and then monacolin J is subsequently performed with the participation of molecular oxygen and P450 monoogygenase mokC (PubMed:19693441). Finally, mokF performs the conversion of monacoline J to monacoline K through the addition of the side-chain diketide moiety (2R)-2-methylbutanoate produced by the diketide synthase mokB (PubMed:19693441). HMG-CoA reductase mokG may act as a down-regulator of monacolin K production (PubMed:18578535). {ECO:0000250|UniProtKB:Q0C8M2, ECO:0000269|PubMed:19968298, ECO:0000303|PubMed:18578535}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); DNA binding (1); Region (3) |
| Keywords | DNA-binding;Metal-binding;Nucleus;Transcription;Transcription regulation;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 49,306 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |