| IED ID | IndEnz0010001083 |
| Enzyme Type ID | esterase001083 |
| Protein Name |
Patatin-17 EC 3.1.1.- |
| Gene Name | |
| Organism | Solanum cardiophyllum (Heartleaf nightshade) (Solanum cardiophyllum Lindl.) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum cardiophyllum (Heartleaf nightshade) (Solanum cardiophyllum Lindl.) |
| Enzyme Sequence | MATTKSFLILIFMILATTSSTFAQLGEMVTVLSIDGGGIRGIIPATILEFLEGQLQEMDNNADARLADYFDVIGGTSTGGLLTAMISTPNENNRPFAAAKEIVPFYFEHGPQIFNPSGQILGPKYDGKYLMQVLQEKLGETRVHQALTEVVISSFDIKTNKPVIFTKSNLANSPELDAKMYDISYSTAAAPTYFPPHYFVTNTSNGDEYEFNLVDGAVATVADPALLSISVATRLAQKDPAFASIRSLNYKKMLLLSLGTGTTSEFDKTYTAKEAATWTAVHWMLVIQKMTDAASSYMTDYYLSTAFQALDSKNNYLRVQENALTGTTTEMDDASEANMELLVQVGENLLKKPVSEDNPETYEEALKRFAKLLSDRKKLRANKASY |
| Enzyme Length | 386 |
| Uniprot Accession Number | Q8LPW4 |
| Absorption | |
| Active Site | ACT_SITE 77; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 215; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Non-specific lipolytic acyl hydrolase (LAH), an activity which is thought to be involved in the response of tubers to pathogens (By similarity). Catalyzes the non-specific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols includng p-nitrophenyl caprate. Confers resistance to southern corn rootworm (SCRW). {ECO:0000250, ECO:0000269|PubMed:12779324}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5-9.5. {ECO:0000269|PubMed:12779324}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (12); Chain (1); Coiled coil (1); Domain (1); Glycosylation (1); Helix (21); Motif (3); Mutagenesis (2); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Coiled coil;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Plant defense;Signal;Storage protein;Vacuole |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1OXW; 4PK9; 4PKA; 4PKB; |
| Mapped Pubmed ID | 25248161; |
| Motif | MOTIF 36..41; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 75..79; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 215..217; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 42,486 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.26 mM for p-nitrophenyl caprate {ECO:0000269|PubMed:12779324}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |