| IED ID | IndEnz0010001088 |
| Enzyme Type ID | esterase001088 |
| Protein Name |
Acetylxylan esterase / glucomannan deacetylase EC 3.1.1.- EC 3.1.1.72 CjCE2B |
| Gene Name | ce2C CJA_3103 |
| Organism | Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Cellvibrionales Cellvibrionaceae Cellvibrio Cellvibrio japonicus (Pseudomonas fluorescens subsp. cellulosa) Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
| Enzyme Sequence | MKPHALIGLLAGMLLSSSLYAADSTKPLPLHIGGRVLVESPANQPVSYTYSWPAVYFETAFKGQSLTLKFDDDQNIFRLIVDDKAPVVINKPGKVDYPVESLAPGKHRVRLEKLTETQSTSGRFLGFYTDPSAKPLALPKRKRQIEFIGDSFTVGYGNTSPSRECTDEELFKTTNSQMAFGPLTAKAFDADYQINASSGFGIVRNYNGTSPDKSLLSLYPYTLNNPDQLYHNKHWKPQVIVIGLGTNDFSTALNDNERWKTREALHADYVANYVKFVKQLHSNNARAQFILMNSDQSNGEIAEQVGKVVAQLKGGGLHQVEQIVFKGLDYSGCHWHPSANDDQLLANLLITHLQQKKGIW |
| Enzyme Length | 360 |
| Uniprot Accession Number | B3PDE5 |
| Absorption | |
| Active Site | ACT_SITE 151; /note=Nucleophile; /evidence=ECO:0000305|PubMed:19338387 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Deacetylation of xylans and xylo-oligosaccharides.; EC=3.1.1.72; Evidence={ECO:0000269|PubMed:19338387}; |
| DNA Binding | |
| EC Number | 3.1.1.-; 3.1.1.72 |
| Enzyme Function | FUNCTION: Involved in the degradation of plant cell wall polysaccharides. Catalyzes the deacetylation of acetylated birchwood xylan and glucomannan, with a large preference for the latter, and of the synthetic substrate 4-nitrophenyl acetate (4-NPAc). {ECO:0000269|PubMed:19338387}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. {ECO:0000269|PubMed:19338387}. |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (16); Chain (1); Helix (12); Signal peptide (1); Site (3); Turn (1) |
| Keywords | 3D-structure;Carbohydrate metabolism;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 2W9X; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,063 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=262 uM for 4-nitrophenyl acetate {ECO:0000269|PubMed:19338387}; KM=4.6 mM for acetylated birchwood xylan {ECO:0000269|PubMed:19338387}; KM=0.84 mM for acetylated glucomannan {ECO:0000269|PubMed:19338387}; Note=kcat is 49820 min(-1) for the deacetylation of 4-nitrophenyl acetate. kcat is 66 min(-1) for the deacetylation of birchwood xylan. kcat is 1348 min(-1) for the deacetylation of glucomannan. {ECO:0000269|PubMed:19338387}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |