| IED ID | IndEnz0010001094 |
| Enzyme Type ID | esterase001094 |
| Protein Name |
Acyl-coenzyme A thioesterase 8 Acyl-CoA thioesterase 8 EC 3.1.2.1 EC 3.1.2.11 EC 3.1.2.2 EC 3.1.2.3 EC 3.1.2.5 Choloyl-coenzyme A thioesterase EC 3.1.2.27 Peroxisomal acyl-coenzyme A thioester hydrolase 1 PTE-1 Peroxisomal long-chain acyl-CoA thioesterase 1 |
| Gene Name | Acot8 Pte1 |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MSKPEDLGDANGDADRGDLSGDLRSVLVTSVLNLEPLDEDLYRGRHYWVPTSQRLFGGQIVGQALVAAAKSVSEDVHVHSLHCYFVRAGDPKVPVLYHVERTRTGASFSVRAVKAVQHGKAIFICQASFQQMQPSPLQHQFSMPTVPPPEELLDHEALIDQYLRDPNLHEKYRVGLNRIAAREVPIEIKLVNPPALNQLQTLEPKQMFWVRARGYIGEGDIKMHCCVAAYISDYAFLGTALLPHQSKYKVNFMVSLDHSMWFHAPFRADHWMLYECESPWAGGSRGLVHGRLWRRDGVLAVTCAQEGVIRSKPRVSESKL |
| Enzyme Length | 320 |
| Uniprot Accession Number | Q8VHK0 |
| Absorption | |
| Active Site | ACT_SITE 233; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 255; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 305; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by CoASH (IC(50)=10-15 uM). Also inhibited by cysteine-reactive agents. {ECO:0000250|UniProtKB:P58137}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=choloyl-CoA + H2O = cholate + CoA + H(+); Xref=Rhea:RHEA:14541, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29747, ChEBI:CHEBI:57287, ChEBI:CHEBI:57373; EC=3.1.2.27; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14542; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=chenodeoxycholoyl-CoA + H2O = chenodeoxycholate + CoA + H(+); Xref=Rhea:RHEA:31511, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:36234, ChEBI:CHEBI:57287, ChEBI:CHEBI:62989; EC=3.1.2.27; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31512; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20290; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=butanoyl-CoA + H2O = butanoate + CoA + H(+); Xref=Rhea:RHEA:40111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:57287, ChEBI:CHEBI:57371; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40112; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=H2O + hexanoyl-CoA = CoA + H(+) + hexanoate; Xref=Rhea:RHEA:40115, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17120, ChEBI:CHEBI:57287, ChEBI:CHEBI:62620; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40116; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=H2O + octanoyl-CoA = CoA + H(+) + octanoate; Xref=Rhea:RHEA:30143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25646, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30144; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=decanoyl-CoA + H2O = CoA + decanoate + H(+); Xref=Rhea:RHEA:40059, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:27689, ChEBI:CHEBI:57287, ChEBI:CHEBI:61430; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40060; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+); Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30136; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate; Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=4,8-dimethylnonanoyl-CoA + H2O = 4,8-dimethylnonanoate + CoA + H(+); Xref=Rhea:RHEA:40223, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:77061, ChEBI:CHEBI:77063; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40224; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=2,6-dimethylheptanoyl-CoA + H2O = 2,6-dimethylheptanoate + CoA + H(+); Xref=Rhea:RHEA:59952, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:84847, ChEBI:CHEBI:143533; Evidence={ECO:0000269|PubMed:11785945};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59953; Evidence={ECO:0000305|PubMed:11785945}; CATALYTIC ACTIVITY: Reaction=H2O + malonyl-CoA = CoA + H(+) + malonate; Xref=Rhea:RHEA:40219, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15792, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40220; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=acetoacetyl-CoA + H2O = acetoacetate + CoA + H(+); Xref=Rhea:RHEA:15673, ChEBI:CHEBI:13705, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57286, ChEBI:CHEBI:57287; EC=3.1.2.11; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15674; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=H2O + propanoyl-CoA = CoA + H(+) + propanoate; Xref=Rhea:RHEA:40103, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17272, ChEBI:CHEBI:57287, ChEBI:CHEBI:57392; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40104; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=H2O + succinyl-CoA = CoA + H(+) + succinate; Xref=Rhea:RHEA:11516, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30031, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292; EC=3.1.2.3; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11517; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=glutaryl-CoA + H2O = CoA + glutarate + H(+); Xref=Rhea:RHEA:40575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30921, ChEBI:CHEBI:57287, ChEBI:CHEBI:57378; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40576; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=H2O + hexanedioyl-CoA = CoA + H(+) + hexanedioate; Xref=Rhea:RHEA:40583, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17128, ChEBI:CHEBI:57287, ChEBI:CHEBI:76327; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40584; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=H2O + octanedioyl-CoA = CoA + H(+) + octanedioate; Xref=Rhea:RHEA:40587, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:76282, ChEBI:CHEBI:76317; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40588; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=decanedioyl-CoA + H2O = CoA + decanedioate + H(+); Xref=Rhea:RHEA:40591, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:76283, ChEBI:CHEBI:76316; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40592; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=dodecanedioyl-CoA + H2O = CoA + dodecanedioate + H(+); Xref=Rhea:RHEA:40595, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:76273, ChEBI:CHEBI:76315; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40596; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=(9Z)-tetradecenoyl-CoA + H2O = (9Z)-tetradecenoate + CoA + H(+); Xref=Rhea:RHEA:40135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32370, ChEBI:CHEBI:57287, ChEBI:CHEBI:65060; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40136; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate; Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=3.1.2.2; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=(9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H(+); Xref=Rhea:RHEA:40131, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:57287, ChEBI:CHEBI:61540; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40132; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate; Xref=Rhea:RHEA:30139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30140; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+); Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40140; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=(9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate + CoA + H(+); Xref=Rhea:RHEA:40143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30245, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40144; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=eicosanoyl-CoA + H2O = CoA + eicosanoate + H(+); Xref=Rhea:RHEA:40147, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32360, ChEBI:CHEBI:57287, ChEBI:CHEBI:57380; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40148; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H(+); Xref=Rhea:RHEA:40151, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32395, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40152; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=(3S)-hydroxy-3-methylglutaryl-CoA + H2O = 3-hydroxy-3-methylglutarate + CoA + H(+); Xref=Rhea:RHEA:16305, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17325, ChEBI:CHEBI:43074, ChEBI:CHEBI:57287; EC=3.1.2.5; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16306; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA + H(+); Xref=Rhea:RHEA:59936, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:63001, ChEBI:CHEBI:85674; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59937; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=2-methyloctadecanoyl-CoA + H2O = 2-methyloctadecanoate + CoA + H(+); Xref=Rhea:RHEA:59940, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:143530, ChEBI:CHEBI:143531; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59941; Evidence={ECO:0000250|UniProtKB:P58137}; CATALYTIC ACTIVITY: Reaction=H2O + prostaglandin F2alpha-CoA = CoA + H(+) + prostaglandin F2alpha; Xref=Rhea:RHEA:59948, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57404, ChEBI:CHEBI:143532; Evidence={ECO:0000250|UniProtKB:P58137};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59949; Evidence={ECO:0000250|UniProtKB:P58137}; |
| DNA Binding | |
| EC Number | 3.1.2.1; 3.1.2.11; 3.1.2.2; 3.1.2.3; 3.1.2.5; 3.1.2.27 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:11785945). Displays no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains (PubMed:11785945). Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity). Is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity). It is involved in the metabolic regulation of peroxisome proliferation (By similarity). {ECO:0000250|UniProtKB:O14734, ECO:0000250|UniProtKB:P58137, ECO:0000269|PubMed:11785945}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Motif (1) |
| Keywords | Fatty acid metabolism;Hydrolase;Lipid metabolism;Peroxisome;Peroxisome biogenesis;Reference proteome;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000269|PubMed:10092594, ECO:0000269|PubMed:11785945}. Note=Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded. {ECO:0000269|PubMed:10092594, ECO:0000269|PubMed:11785945}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 318..320; /note=Microbody targeting signal; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 36,003 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:14541; RHEA:14542; RHEA:31511; RHEA:31512; RHEA:20289; RHEA:20290; RHEA:40111; RHEA:40112; RHEA:40115; RHEA:40116; RHEA:30143; RHEA:30144; RHEA:40059; RHEA:40060; RHEA:30135; RHEA:30136; RHEA:40119; RHEA:40120; RHEA:40223; RHEA:40224; RHEA:59952; RHEA:59953; RHEA:40219; RHEA:40220; RHEA:15673; RHEA:15674; RHEA:40103; RHEA:40104; RHEA:11516; RHEA:11517; RHEA:40575; RHEA:40576; RHEA:40583; RHEA:40584; RHEA:40587; RHEA:40588; RHEA:40591; RHEA:40592; RHEA:40595; RHEA:40596; RHEA:40135; RHEA:40136; RHEA:16645; RHEA:16646; RHEA:40131; RHEA:40132; RHEA:30139; RHEA:30140; RHEA:40139; RHEA:40140; RHEA:40143; RHEA:40144; RHEA:40147; RHEA:40148; RHEA:40151; RHEA:40152; RHEA:16305; RHEA:16306; RHEA:59936; RHEA:59937; RHEA:59940; RHEA:59941; RHEA:59948; RHEA:59949 |
| Cross Reference Brenda | 3.1.2.2;3.1.2.20; |