Detail Information for IndEnz0010001095
IED ID IndEnz0010001095
Enzyme Type ID esterase001095
Protein Name GDSL esterase/lipase ACHE
EC 3.1.1.-
Acetylcholinesterase
AChE
Gene Name ACHE ZEAMMB73_Zm00001d021961 Zm00014a_018493
Organism Zea mays (Maize)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae PACMAD clade Panicoideae Andropogonodae Andropogoneae Tripsacinae Zea Zea mays (Maize)
Enzyme Sequence MATAATATAGSRAAVLLLLSLALALALRPSDAGAGGDCHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTFFGMPAGRYCDGRLVIDFIAESLGLTHLSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFLSGFSPISLDVQFWEFEQFINRSQLVYNNKGGIYREILPRAEYFSQALYTFDIGQNDITSSYFVNNTTEEVEAIIPDLMERLTSIIQSVYSRGGRYFWIHNTGPLGCLPYALLHRPDLAIPADGTGCSVTYNKVAQLFNLRLKETVASLRKTHPDAAFTYVDVYTAKYKLISQANKLGFDDPLLTCCGYGGGRYNLDLSVGCGGKKQVNGTSVVVGKSCENPSKRVSWDGVHFTEAANKFVFDQIVAGALSDPPVALRQACHSRGQ
Enzyme Length 396
Uniprot Accession Number B4FZ87
Absorption
Active Site ACT_SITE 49; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P0ADA1; ACT_SITE 359; /evidence=ECO:0000250|UniProtKB:P0ADA1; ACT_SITE 362; /evidence=ECO:0000250|UniProtKB:P0ADA1
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Esterase that can hydrolyze acetylthiocholine and propionylthiocholine in vitro (PubMed:15980188). Substrate preference is propionylthiocholine > acetylthiocholine (PubMed:15980188). Possesses extremely low activity against butyrylthiocholine (PubMed:15980188). {ECO:0000269|PubMed:15980188}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Glycosylation (5); Sequence conflict (4); Signal peptide (1)
Keywords Direct protein sequencing;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Secreted;Serine esterase;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..31; /evidence=ECO:0000269|PubMed:15980188
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 42,680
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4.7 mM for acetylthiocholine {ECO:0000269|PubMed:15980188}; KM=3.1 mM for propionylthiocholine {ECO:0000269|PubMed:15980188}; Vmax=1.7 umol/min/mg enzyme with acetylthiocholine as substrate {ECO:0000269|PubMed:15980188}; Vmax=1.7 umol/min/mg enzyme with propionylthiocholine as substrate {ECO:0000269|PubMed:15980188};
Metal Binding
Rhea ID
Cross Reference Brenda