Detail Information for IndEnz0010001101
IED ID IndEnz0010001101
Enzyme Type ID esterase001101
Protein Name Acyl-protein thioesterase 1
EC 3.1.2.-
Palmitoyl-protein hydrolase
EC 3.1.2.22
Gene Name FGRAMPH1_01T20223 FGRRES_06404_M FGSG_06404
Organism Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Nectriaceae Fusarium Fusarium sambucinum species complex Gibberella zeae (Wheat head blight fungus) (Fusarium graminearum) Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum)
Enzyme Sequence MSSGRIAPLVFPAASQHTATVIFVHGLGDTGHGWASAVENWRRRQKMDEVKFILPHAPQIPISVNMGMRMPGWFDIKQLGGDVDSLIRNEDTEGIKLSQKYFHNLIQQEIDSGIVPERIVLGGFSQGGAMSLLAGLTCTSKLGGILGLSSWLLLSKTFADMVKPTDANRQTPVMMFHGEEDPIVPCERGKLSAELLKGLGYDVAWKTYPGMGHSAVPEELDEVEAFLRKQLPPKN
Enzyme Length 235
Uniprot Accession Number Q4I8Q4
Absorption
Active Site ACT_SITE 125; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 181; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 213; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=H2O + S-hexadecanoyl-L-cysteinyl-[protein] = H(+) + hexadecanoate + L-cysteinyl-[protein]; Xref=Rhea:RHEA:19233, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:74151; EC=3.1.2.22; Evidence={ECO:0000250|UniProtKB:Q12354};
DNA Binding
EC Number 3.1.2.-; 3.1.2.22
Enzyme Function FUNCTION: Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo. {ECO:0000250|UniProtKB:Q12354}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Erroneous initiation (1)
Keywords Cytoplasm;Fatty acid metabolism;Hydrolase;Lipid metabolism;Nucleus;Reference proteome;Serine esterase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q12354}. Nucleus {ECO:0000250|UniProtKB:Q12354}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 25,765
Kinetics
Metal Binding
Rhea ID RHEA:19233
Cross Reference Brenda