| IED ID | IndEnz0010001102 |
| Enzyme Type ID | esterase001102 |
| Protein Name |
Chitin deacetylase 8 BmCDA8 EC 3.5.1.41 Chitin deacetylase 17 |
| Gene Name | CDA8 |
| Organism | Bombyx mori (Silk moth) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Amphiesmenoptera Lepidoptera (butterflies and moths) Glossata Neolepidoptera Heteroneura Ditrysia Obtectomera Bombycoidea (hawk-moths) Bombycidae (silkworm moths) Bombycinae Bombyx Bombyx mori (Silk moth) |
| Enzyme Sequence | MKRLSVLCSLLLVAAALGTELPLATPCDEEACKLPDCRCSSTNIPGGLRARDTPQFVTVTFDDGINVINIETYREVLYGRSNSNRCPAGATFYVSHEYTNYQLVNELYNRGFEIALHSISHRTPQAFWADATYQNLVQEIGDQKRQMAHFASIPASAIKGVRIPFLQMSGNTSFQVMADFDLLYDCTWPTTALTNPGLWPYTLHHESIQDCIIPPCPTASIPGPWVLPMISWRDLNNFPCSMVDGCFFTPDRTDEEGWFKFILTNFERHYLGNRAPFGFFVHEWFISSNPAIKRAFVRFMDIINNLNDVFMVNSAEVIDWVKNPVPIDRYRQQQCKFTMPSICRPSFCGPLTGTHNQLSYYMTICNTCPRNYPWVGNPLGQ |
| Enzyme Length | 381 |
| Uniprot Accession Number | H9JW43 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-N-acetyl-beta-D-glucosaminyl](n) + n H2O = n acetate + chitosan; Xref=Rhea:RHEA:10464, Rhea:RHEA-COMP:9593, Rhea:RHEA-COMP:9597, ChEBI:CHEBI:15377, ChEBI:CHEBI:17029, ChEBI:CHEBI:30089, ChEBI:CHEBI:57704; EC=3.5.1.41; Evidence={ECO:0000269|PubMed:30755482}; |
| DNA Binding | |
| EC Number | 3.5.1.41 |
| Enzyme Function | FUNCTION: Hydrolyzes the N-acetamido groups of N-acetyl-D-glucosamine (GlcNAc) residues in chitin. Shows activity towards the chitinous oligomers GlcNAc(3), GlcNAc(4), GlcNAc(5) and GlcNAc(6), but not GlcNAc or GlcNAc(2). Requires the substrate to occupy subsites 0, +1, and +2 for optimum catalysis. {ECO:0000269|PubMed:30755482}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (7); Glycosylation (1); Metal binding (3); Mutagenesis (2); Signal peptide (1) |
| Keywords | 3D-structure;Carbohydrate metabolism;Chitin degradation;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Polysaccharide degradation;Reference proteome;Secreted;Signal;Zinc |
| Interact With | |
| Induction | INDUCTION: Strongly down-regulated in response to 20-hydroxyecdysone (20E). Up-regulated in response to juvenile hormone analog. {ECO:0000269|PubMed:30987273}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 5Z34; |
| Mapped Pubmed ID | 29251378; |
| Motif | |
| Gene Encoded By | |
| Mass | 43,310 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=76.7 mM for GlcNAc(3) {ECO:0000269|PubMed:30755482}; KM=12.3 mM for GlcNAc(4) {ECO:0000269|PubMed:30755482}; KM=15.7 mM for GlcNAc(5) {ECO:0000269|PubMed:30755482}; KM=9.2 mM for GlcNAc(6) {ECO:0000269|PubMed:30755482}; KM=1.926 mg/ml for ethylene glycol chitin {ECO:0000269|PubMed:30755482}; KM=1.599 mg/ml for colloidal chitin {ECO:0000269|PubMed:30755482}; Note=kcat is 7.62 min(-1) for GlcNAc(3) (PubMed:30755482). kcat is 5.59 min(-1) for GlcNAc(4) (PubMed:30755482). kcat is 7.52 min(-1) for GlcNAc(5) (PubMed:30755482). kcat is 9.07 min(-1) for GlcNAc(6) (PubMed:30755482). kcat is 0.097 min(-1) for ethylene glycol chitin (PubMed:30755482). kcat is 0.012 min(-1) for colloidal chitin (PubMed:30755482). {ECO:0000269|PubMed:30755482}; |
| Metal Binding | METAL 63; /note="Zinc"; /evidence="ECO:0000269|PubMed:30755482, ECO:0007744|PDB:5Z34"; METAL 117; /note="Zinc; via tele nitrogen"; /evidence="ECO:0000269|PubMed:30755482, ECO:0007744|PDB:5Z34"; METAL 121; /note="Zinc; via tele nitrogen"; /evidence="ECO:0000269|PubMed:30755482, ECO:0007744|PDB:5Z34" |
| Rhea ID | RHEA:10464 |
| Cross Reference Brenda | 3.5.1.41; |