Detail Information for IndEnz0010001103
IED ID IndEnz0010001103
Enzyme Type ID esterase001103
Protein Name Cutinase 1
EC 3.1.1.74
Cutin hydrolase 1
Fragment
Gene Name
Organism Colletotrichum kahawae (Coffee berry disease fungus)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Glomerellales Glomerellaceae Colletotrichum Colletotrichum gloeosporioides species complex Colletotrichum kahawae (Coffee berry disease fungus)
Enzyme Sequence VIYIFAR
Enzyme Length 7
Uniprot Accession Number P86010
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Inhibited by diisopropyl fluorophosphate (DFP). {ECO:0000269|PubMed:17043825}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Cutin + H(2)O = cutin monomers.; EC=3.1.1.74; Evidence={ECO:0000255|PROSITE-ProRule:PRU10108, ECO:0000255|PROSITE-ProRule:PRU10109, ECO:0000305|PubMed:17043825};
DNA Binding
EC Number 3.1.1.74
Enzyme Function FUNCTION: Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants (PubMed:17043825). Degrades cutin, a macromolecule that forms the structure of the plant cuticle (PubMed:17043825). Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of fungal infection (By similarity). {ECO:0000250|UniProtKB:P00590, ECO:0000269|PubMed:17043825}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Non-terminal residue (2)
Keywords Direct protein sequencing;Hydrolase;Secreted;Serine esterase;Virulence
Interact With
Induction INDUCTION: By contact with cutin. {ECO:0000269|PubMed:17043825}.
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17043825}.
Modified Residue
Post Translational Modification PTM: The N-terminus is blocked. {ECO:0000269|PubMed:17043825}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 881
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda