IED ID | IndEnz0010001105 |
Enzyme Type ID | esterase001105 |
Protein Name |
Teichoic acid D-alanine hydrolase EC 3.1.1.103 Teichoic acid D-alanine esterase |
Gene Name | fmtA fmt SACOL1066 |
Organism | Staphylococcus aureus (strain COL) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus aureus Staphylococcus aureus (strain COL) |
Enzyme Sequence | MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETKLSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKMSKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFKTSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLVLAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRPNILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFYAKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTKGVIVQ |
Enzyme Length | 397 |
Uniprot Accession Number | Q5HH27 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=((4-D-Ala)-(2-GlcNAc)-Rib-ol-P)(n)-(Gro-P)(m)-beta-D-ManNAc-(1->4)-alpha-D-GlcNAc-P-peptidoglycan + n H(2)O = ((2-GlcNAc)-Rib-ol-P)(n)-(Gro-P)(m)-beta-D-ManNAc-(1->4)-alpha-D-GlcNAc-P-peptidoglycan + n D-alanine.; EC=3.1.1.103; Evidence={ECO:0000250|UniProtKB:Q7A2T0}; |
DNA Binding | |
EC Number | 3.1.1.103 |
Enzyme Function | FUNCTION: Catalyzes the liberation of D-alanyl moieties present on wall teichoic acid (WTA) and lipoteichoic acid (LTA) (By similarity). Affects the methicillin resistance level and autolysis in the presence of Triton X-100 as well as the cell wall structure (PubMed:9371333, PubMed:10471551). {ECO:0000250|UniProtKB:Q7A2T0, ECO:0000269|PubMed:10471551, ECO:0000269|PubMed:9371333}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Chain (1); Signal peptide (1) |
Keywords | Antibiotic resistance;Cell membrane;Cell wall biogenesis/degradation;Hydrolase;Membrane;Signal |
Interact With | |
Induction | INDUCTION: Transcription is dose dependently increased by the addition of beta-lactam antibiotics, fosfomycin, and bacitracin. |
Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:10471551}; Peripheral membrane protein {ECO:0000269|PubMed:10471551}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 46,067 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |