| IED ID | IndEnz0010001109 |
| Enzyme Type ID | esterase001109 |
| Protein Name |
Ferri-bacillibactin esterase BesA EC 3.1.-.- Bacillibactin trilactone hydrolase |
| Gene Name | besA yuiI BSU32010 |
| Organism | Bacillus subtilis (strain 168) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
| Enzyme Sequence | MKEQTTDRTNGGTSNAFTIPGTEVRMMSSRNENRTYHIFISKPSTPPPPAGYPVIYLLDANSVFGTMTEAVRIQGRRPEKTGVIPAVIVGIGYETAEPFSSARHRDFTMPTAQSKLPERPDGREWPEHGGAEGFFRFIEEDLKPEIERDYQIDKKRQTIFGHSLGGLFVLQVLLTKPDAFQTYIAGSPSIHWNKPFILKKTDHFVSLTKKNNQPINILLAAGELEQHHKSRMNDNARELYERLAVLSEQGIRAEFCEFSGEGHISVLPVLVSRALRFALHPDGPHLSMG |
| Enzyme Length | 289 |
| Uniprot Accession Number | O32102 |
| Absorption | |
| Active Site | ACT_SITE 163; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 225; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 263; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.-.- |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of the trilactone cycle of ferri-bacillibactin (ferri-BB) complex, leading to the formation of bacillibactin monomers and to cytosolic iron release, thus making iron available for metabolic use. Can also hydrolyze bacillibactin (BB), however the catalytic efficiency for ferri-BB hydrolysis is much higher than for BB. {ECO:0000269|PubMed:16889643}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1) |
| Keywords | Cytoplasm;Hydrolase;Reference proteome;Serine esterase |
| Interact With | |
| Induction | INDUCTION: Repressed by fur in the presence of iron. {ECO:0000269|PubMed:12354229}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:16889643}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 32,439 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.54 uM for ferri-bacillibactin complex {ECO:0000269|PubMed:16889643}; KM=24.4 uM for bacillibactin {ECO:0000269|PubMed:16889643}; KM=221 uM for enterobactin {ECO:0000269|PubMed:16889643}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |