Detail Information for IndEnz0010001110
IED ID IndEnz0010001110
Enzyme Type ID esterase001110
Protein Name Pimeloyl-
acyl-carrier protein methyl ester esterase
EC 3.1.1.85
Biotin synthesis protein BioH
Carboxylesterase BioH
Gene Name bioH SEN3335
Organism Salmonella enteritidis PT4 (strain P125109)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella enteritidis Salmonella enteritidis PT4 (strain P125109)
Enzyme Sequence MNDIWWQTYGEGNCHLVLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMTLEEMTAQVAKNAPDQAIWLGWSLGGLVASQMALTHPERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDARTLKSVVLAQPMPDVEVLNGGLEILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIAPLLDTLWPHSTSQIMAKAAHAPFISHPAAFCQALMTLKSSL
Enzyme Length 256
Uniprot Accession Number B5R369
Absorption
Active Site ACT_SITE 82; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 207; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 235; /evidence=ECO:0000255|HAMAP-Rule:MF_01260
Activity Regulation
Binding Site BINDING 22; /note=Substrate; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; BINDING 235; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_01260
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6-carboxyhexanoyl-[ACP] + H(+) + methanol; Xref=Rhea:RHEA:42700, Rhea:RHEA-COMP:9955, Rhea:RHEA-COMP:10186, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:78846, ChEBI:CHEBI:82735; EC=3.1.1.85; Evidence={ECO:0000255|HAMAP-Rule:MF_01260};
DNA Binding
EC Number 3.1.1.85
Enzyme Function FUNCTION: The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters. {ECO:0000255|HAMAP-Rule:MF_01260}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Cofactor biosynthesis; biotin biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01260}.
nucleotide Binding
Features Active site (3); Binding site (2); Chain (1); Domain (1); Region (2)
Keywords Biotin biosynthesis;Cytoplasm;Hydrolase;Serine esterase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01260}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 28,241
Kinetics
Metal Binding
Rhea ID RHEA:42700
Cross Reference Brenda