Detail Information for IndEnz0010001112
IED ID IndEnz0010001112
Enzyme Type ID esterase001112
Protein Name Acetyl esterase Axe7A
EC 3.1.1.-
Gene Name axe7A PRU_2678
Organism Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)
Taxonomic Lineage cellular organisms Bacteria FCB group Bacteroidetes/Chlorobi group Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella Prevotella ruminicola (Bacteroides ruminicola) Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)
Enzyme Sequence MFNFAPKQTTEMKKLLFTLVFVLGSMATALAENYPYRADYLWLTVPNHADWLYKTGERAKVEVSFCLYGMPQNVEVAYEIGPDMMPATSSGKVTLKNGRAVIDMGTMKKPGFLDMRLSVDGKYQHHVKVGFSPELLKPYTKNPQDFDAFWKANLDEARKTPVSVSCNKVDKYTTDAFDCYLLKIKTDRRHSIYGYLTKPKKAGKYPVVLCPPGAGIKTIKEPMRSTFYAKNGFIRLEMEIHGLNPEMTDEQFKEITTAFDYENGYLTNGLDDRDNYYMKHVYVACVRAIDYLTSLPDWDGKNVFVQGGSQGGALSLVTAGLDPRVTACVANHPALSDMAGYLDNRAGGYPHFNRLKNMFTPEKVNTMAYYDVVNFARRITCPVYITWGYNDNVCPPTTSYIVWNLITAPKESLITPINEHWTTSETNYTQMLWLKKQVK
Enzyme Length 439
Uniprot Accession Number D5EXI2
Absorption
Active Site ACT_SITE 309; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 391; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 420; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Involved in degradation of plant cell wall polysaccharides. Has acetyl esterase activity towards a broad range of substrates including xylose-tetraacetate, 4-O-methylumbelliferyl acetate, glucose-pentaacetate, cephalosporin C, and acetylated xylo-oligosaccharides smaller than xylo-heptaose. Displays no detectable activity on polymeric acetylated xylan. {ECO:0000269|PubMed:21742923}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 40 degrees Celsius with glucose-pentaacetate as substrate. Active from 30 to 50 degrees Celsius. At temperatures of 30 degrees Celsius and lower, less than 10% of the maximum activity is left. At 50 degrees Celsius, retains 60% of its maximum activity. {ECO:0000269|PubMed:21742923};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0 with glucose-pentaacetate as substrate. Active from pH 5.0 to 8.0. {ECO:0000269|PubMed:21742923};
Pathway PATHWAY: Glycan degradation; xylan degradation. {ECO:0000269|PubMed:21742923}.
nucleotide Binding
Features Active site (3); Chain (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Hydrolase;Polysaccharide degradation;Reference proteome;Serine esterase;Signal;Xylan degradation
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..31; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 49,867
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda