Detail Information for IndEnz0010001113
IED ID IndEnz0010001113
Enzyme Type ID esterase001113
Protein Name Carbohydrate acetyl esterase/feruloyl esterase
Includes: Carbohydrate acetyl esterase
EC 3.1.1.-
; Feruloyl esterase
EC 3.1.1.73
Ferulic acid esterase
Gene Name axe1-6A PRU_2707
Organism Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)
Taxonomic Lineage cellular organisms Bacteria FCB group Bacteroidetes/Chlorobi group Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella Prevotella ruminicola (Bacteroides ruminicola) Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)
Enzyme Sequence MYQSTLKTILLASALLILPASMSAQKRKAAPKKAATEQVGKPDPNFYIFLCFGQSNMEGNARPEAQDLTSPGPRFLLMPAVDFPEKGRKMGEWCEASAPLCRPNTGLTPADWFGRTLVASLPENIKIGVIHVAIGGIDIKGFLPDSIQNYLKVAPNWMKGMLAAYDNNPYERLVTLAKKAQKDGVIKGILMHQGETNTGDPKWAGMVKQVYDNLCGDLNLKPEEVNLYAGNIVQADGKGVCIGCKKQIDELPLTLHTSQVISSDGCTNGPDRLHFDAAGYRELGCRYGEAVARHLGYEPKRPYIEMPKQIEVPADAFIAETTVPGNEFPKVDKEGRAYFRIAAPEARKVVLDICNKKYDMQRDGKGNFMAVTDPLPVGFHYYFLNINGVNFIDPSTETFFGCNRESGGIEIPEGSEGDYYRPQQGVPAGQVRSIYYYSNEQQTWRHAMVYTPAEYELAKNAKKRYPVLYLQHGMGEDETGWSKQGHMQHIMDNAIAKGEAVPMIVVMESGDIKAPFGGGNNQAGRSAYGASFYPVLLNDLIPYIDSNYRTKSDRENRAMAGLSWGGHQTFDVVLTNLDKFAWLGTFSGAIFGLDVKTAYDGVFANADEFNKKIHYMYMNWGEEDFIKSGDIVKQLRELGIKVDSNESKGTAHEWLTWRRGLNEFIPHLFKK
Enzyme Length 671
Uniprot Accession Number D5EXZ4
Absorption
Active Site ACT_SITE 55; /note=For acetyl esterase activity; /evidence=ECO:0000250; ACT_SITE 271; /note=For acetyl esterase activity; /evidence=ECO:0000250; ACT_SITE 274; /note=For acetyl esterase activity; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide.; EC=3.1.1.73; Evidence={ECO:0000269|PubMed:21742923};
DNA Binding
EC Number 3.1.1.-; 3.1.1.73
Enzyme Function FUNCTION: Involved in degradation of plant cell wall polysaccharides. Bifunctional esterase that possesses both acetyl esterase and ferulic acid esterase activities. Has deacetylase activity towards acetylated xylo-oligosaccharides smaller than xylo-heptaose, as well as from glucose-pentaacetate. Is also able to release ferulic acid from methylferulate, and from the more natural substrates wheat bran, corn fiber, and XOS(FA,Ac), a corn fiber-derived substrate enriched in O-acetyl and ferulic acid esters. {ECO:0000269|PubMed:21742923}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 35 degrees Celsius with glucose-pentaacetate as substrate. Active from 20 to 50 degrees Celsius. Still exhibits 40 to 70% of the maximum activity after 20 hours of incubation at 50 degrees Celsius. {ECO:0000269|PubMed:21742923};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.5 with glucose-pentaacetate as substrate. Active from pH 5.0 to 8.0. {ECO:0000269|PubMed:21742923};
Pathway PATHWAY: Glycan degradation; xylan degradation. {ECO:0000269|PubMed:21742923}.
nucleotide Binding
Features Active site (3); Chain (1); Region (2); Signal peptide (1)
Keywords Carbohydrate metabolism;Hydrolase;Multifunctional enzyme;Polysaccharide degradation;Reference proteome;Signal;Xylan degradation
Interact With
Induction INDUCTION: By growth on ester-enriched corn oligosaccharides. {ECO:0000269|PubMed:21742923}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..24; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 74,683
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda