Detail Information for IndEnz0010001116
IED ID IndEnz0010001116
Enzyme Type ID esterase001116
Protein Name N-acetylneuraminate 9-O-acetyltransferase
EC 2.3.1.45
CAS1 domain-containing protein 1
Sialate O-acetyltransferase
SOAT
Gene Name CASD1 C7orf12 Nbla04196
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MAALAYNLGKREINHYFSVRSAKVLALVAVLLLAACHLASRRYRGNDSCEYLLSSGRFLGEKVWQPHSCMMHKYKISEAKNCLVDKHIAFIGDSRIRQLFYSFVKIINPQFKEEGNKHENIPFEDKTASVKVDFLWHPEVNGSMKQCIKVWTEDSIAKPHVIVAGAATWSIKIHNGSSEALSQYKMNITSIAPLLEKLAKTSDVYWVLQDPVYEDLLSENRKMITNEKIDAYNEAAVSILNSSTRNSKSNVKMFSVSKLIAQETIMESLDGLHLPESSRETTAMILMNVYCNKILKPVDGSCCQPRPPVTLIQKLAACFFTLSIIGYLIFYIIHRNAHRKNKPCTDLESGEEKKNIINTPVSSLEILLQSFCKLGLIMAYFYMCDRANLFMKENKFYTHSSFFIPIIYILVLGVFYNENTKETKVLNREQTDEWKGWMQLVILIYHISGASTFLPVYMHIRVLVAAYLFQTGYGHFSYFWIKGDFGIYRVCQVLFRLNFLVVVLCIVMDRPYQFYYFVPLVTVWFMVIYVTLALWPQIIQKKANGNCFWHFGLLLKLGFLLLFICFLAYSQGAFEKIFSLWPLSKCFELKGNVYEWWFRWRLDRYVVFHGMLFAFIYLALQKRQILSEGKGEPLFSNKISNFLLFISVVSFLTYSIWASSCKNKAECNELHPSVSVVQILAFILIRNIPGYARSVYSSFFAWFGKISLELFICQYHIWLAADTRGILVLIPGNPMLNIIVSTFIFVCVAHEISQITNDLAQIIIPKDNSSLLKRLACIAAFFCGLLILSSIQDKSKH
Enzyme Length 797
Uniprot Accession Number Q96PB1
Absorption
Active Site ACT_SITE 94; /note=Acyl-ester intermediate; /evidence=ECO:0000269|PubMed:26169044; ACT_SITE 270; /evidence=ECO:0000305|PubMed:26169044; ACT_SITE 273; /evidence=ECO:0000305|PubMed:26169044
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=acetyl-CoA + N-acetylneuraminate = CoA + N-acetyl-9-O-acetylneuraminate; Xref=Rhea:RHEA:26043, ChEBI:CHEBI:28999, ChEBI:CHEBI:35418, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.45; Evidence={ECO:0000269|PubMed:26169044}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + N-acetylneuraminate = CoA + N-acetyl-7-O-acetylneuraminate; Xref=Rhea:RHEA:20808, ChEBI:CHEBI:28944, ChEBI:CHEBI:35418, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.45; Evidence={ECO:0000269|PubMed:26169044};
DNA Binding
EC Number 2.3.1.45
Enzyme Function FUNCTION: O-acetyltransferase that catalyzes 9-O-acetylation of sialic acids (PubMed:20947662, PubMed:26169044). Sialic acids are sugars at the reducing end of glycoproteins and glycolipids, and are involved in various processes such as cell-cell interactions, host-pathogen recognition (PubMed:20947662, PubMed:26169044). {ECO:0000269|PubMed:26169044, ECO:0000305|PubMed:20947662}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Erroneous gene model prediction (1); Erroneous initiation (1); Frameshift (2); Glycosylation (3); Mutagenesis (1); Natural variant (1); Sequence conflict (1); Topological domain (16); Transmembrane (15)
Keywords Acyltransferase;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:26169044}; Multi-pass membrane protein {ECO:0000305}.
Modified Residue
Post Translational Modification PTM: N-glycosylated. {ECO:0000269|PubMed:26169044}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 91,680
Kinetics
Metal Binding
Rhea ID RHEA:26043; RHEA:20808
Cross Reference Brenda 2.3.1.45;