Detail Information for IndEnz0010001118
IED ID IndEnz0010001118
Enzyme Type ID esterase001118
Protein Name Rhodomycin D methylesterase DnrP
EC 3.1.1.-
10-carbomethoxy-13-deoxycarminomycin esterase
4-O-methylrhodomycin D methylesterase
Gene Name dnrP
Organism Streptomyces peucetius
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces peucetius
Enzyme Sequence MPTRMITNDEVTLWSEGLGDPADAPLLLIAGGNLSAKSWPDEFVERLVAAGHFVIRYDHRDTGRSSRCDFALHPYGFDELAADALAVLDGWQVRAAHVVGMSLGNTIGQLLALDAPERLLTLTVMLGGALDVDFDADLEAALKGEPSVSGLPVPSQRFLDMMTMLQQPAETDEELLERRVEKWRLLNGEGVPFDADEFRRRELLAAGHAGTFDEPIVHHMIPLPPVSRGAELSRVTTPVLAIQAMCDPAAPPPHARHLANRIPGARVVEIENMGHALPLAVHEPLAAAICAHTRAATV
Enzyme Length 298
Uniprot Accession Number Q54809
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=H2O + rhodomycin D = 10-carboxy-13-deoxycarminomycin + H(+) + methanol; Xref=Rhea:RHEA:40247, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:77073, ChEBI:CHEBI:77077; CATALYTIC ACTIVITY: Reaction=4-O-methylrhodomycin D + H2O = 10-carboxy-13-deoxydaunorubicin + H(+) + methanol; Xref=Rhea:RHEA:40251, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:77074, ChEBI:CHEBI:77076;
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. Catalyzes the removal of methyl group from the carbomethoxy group of rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 4-O-methylrhodomycin D to yield 10-carboxy-13-deoxycarminomycin and 10-carboxy-13-deoxydaunorubicin, respectively. Could be also involved in the decarboxylation of 10-carboxy-13-deoxycarminomycin and 10-carboxy-13-deoxydaunorubicin to yield 13-deoxycarminomycin and 13-deoxydaunorubicin, respectively. It seems that DnrK may influence the ability of DnrP to carry out the decarboxylation (Probable). {ECO:0000305|PubMed:7601857}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Antibiotic biosynthesis; daunorubicin biosynthesis.; PATHWAY: Antibiotic biosynthesis; carminomycin biosynthesis.
nucleotide Binding
Features Chain (1); Domain (1)
Keywords Antibiotic biosynthesis;Hydrolase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 32,245
Kinetics
Metal Binding
Rhea ID RHEA:40247; RHEA:40251
Cross Reference Brenda