| IED ID | IndEnz0010001119 |
| Enzyme Type ID | esterase001119 |
| Protein Name |
Rhodomycin D methylesterase DauP EC 3.1.1.- 10-carbomethoxy-13-deoxycarminomycin esterase 4-O-methylrhodomycin D methylesterase |
| Gene Name | dauP |
| Organism | Streptomyces sp. (strain C5) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces unclassified Streptomyces Streptomyces sp. (strain C5) |
| Enzyme Sequence | MPTRMITKDEVTLWSEGIGDPADAPLLLIAGGNLSARSWPDEFVERLAAAGHFVIRYDHRDTGRSSRYDFALHPYGFDELATDALAVLDAWQVRAAHVVGMSLGNTIGQLLALDAPERLLTLTVMLGGALDVDFDADLEAALKGEPSVSGLPVPSRRFLDMMMLLQQPAGTDEELLERRVEKWRLLNGEGVPFDSDEFRRRELLAAGHAGTFDEPIVHHMIPQPPVSRGAELARITTPVLAIQAMCDPAAPPPHARHLADRIPGARVVEIENMGHALPLAVHEPLAAAICAHTRAATV |
| Enzyme Length | 298 |
| Uniprot Accession Number | Q55217 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + rhodomycin D = 10-carboxy-13-deoxycarminomycin + H(+) + methanol; Xref=Rhea:RHEA:40247, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:77073, ChEBI:CHEBI:77077; Evidence={ECO:0000269|PubMed:9098063}; CATALYTIC ACTIVITY: Reaction=4-O-methylrhodomycin D + H2O = 10-carboxy-13-deoxydaunorubicin + H(+) + methanol; Xref=Rhea:RHEA:40251, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:77074, ChEBI:CHEBI:77076; Evidence={ECO:0000269|PubMed:9098063}; |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. Catalyzes the removal of methyl group from the carbomethoxy group of rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 4-O-methylrhodomycin D to yield 10-carboxy-13-deoxycarminomycin and 10-carboxy-13-deoxydaunorubicin, respectively. Could be also involved in the decarboxylation of 10-carboxy-13-deoxycarminomycin and 10-carboxy-13-deoxydaunorubicin to yield 13-deoxycarminomycin and 13-deoxydaunorubicin, respectively. It seems that DauK may influence the ability of DauP to carry out the decarboxylation. {ECO:0000269|PubMed:9098063}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Antibiotic biosynthesis; daunorubicin biosynthesis.; PATHWAY: Antibiotic biosynthesis; carminomycin biosynthesis. |
| nucleotide Binding | |
| Features | Chain (1); Domain (1) |
| Keywords | Antibiotic biosynthesis;Hydrolase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 32,361 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:40247; RHEA:40251 |
| Cross Reference Brenda |