IED ID | IndEnz0010001123 |
Enzyme Type ID | esterase001123 |
Protein Name |
Ethanol acetyltransferase 1 EC 2.3.1.268 Acetyl-CoA hydrolase EC 3.1.2.1 Acetyl-CoA thioesterase Alcohol acetyltransferase AAT Ethyl acetate esterase EC 3.1.1.- |
Gene Name | EAT1 BON22_2185 CYFA0S_05e01970g |
Organism | Cyberlindnera fabianii (Yeast) (Hansenula fabianii) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Phaffomycetaceae Cyberlindnera Cyberlindnera fabianii (Yeast) (Hansenula fabianii) |
Enzyme Sequence | MFKPTRVLKSSQPILNSLPHAETVKMAYDLHLPKKTLHQNMNITSDEPIVFVHGIFGSKKSYATDSKLIANGTHSPVYTIDLRNHGETGHAQPFNYDTLVQDIKEFCSTHNLSNIKLVGYSLGAKVSMLAALRLPELVKSAVIIDNAPIKQPYIESYMKQYIKSMLHVDDAKISTTDKDWKRKASEAMKRYMPNATVRKNLLVNLVNKKPEGFESPAIDFENGNIQFLNPIKHMEEMAVKDVSDWPVESTEGLKFDGPVKFIRGLKSPFISPEGFKKINEHFPKNEFYDVNSAHDILDQRPSEYVKVICDFFNLQRYNSAPAHTVLGHKAPEMRV |
Enzyme Length | 335 |
Uniprot Accession Number | A0A061B0Q2 |
Absorption | |
Active Site | ACT_SITE 121; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6; ACT_SITE 145; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6; ACT_SITE 294; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetyl-CoA + ethanol = CoA + ethyl acetate; Xref=Rhea:RHEA:55972, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.268; Evidence={ECO:0000269|PubMed:28356220}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1; Evidence={ECO:0000250|UniProtKB:A0A1E3P8S6}; CATALYTIC ACTIVITY: Reaction=ethyl acetate + H2O = acetate + ethanol + H(+); Xref=Rhea:RHEA:58148, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:30089; Evidence={ECO:0000250|UniProtKB:A0A1E3P8S6}; |
DNA Binding | |
EC Number | 2.3.1.268; 3.1.2.1; 3.1.1.- |
Enzyme Function | FUNCTION: Alcohol acetyltransferase that catalyzes the synthesis of ethyl acetate from ethanol and acetyl-CoA (PubMed:28356220). Can also function as a thioesterase by hydrolyzing acetyl-CoA in the absence of ethanol, as well as esterase hydrolyzing ethyl acetate (By similarity). {ECO:0000250|UniProtKB:A0A1E3P8S6, ECO:0000269|PubMed:28356220}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Chain (1); Domain (1) |
Keywords | Hydrolase;Mitochondrion;Reference proteome;Transferase |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:A0A1E3P8S6}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 38,016 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:55972; RHEA:20289; RHEA:58148 |
Cross Reference Brenda |