Detail Information for IndEnz0010001130
IED ID IndEnz0010001130
Enzyme Type ID esterase001130
Protein Name Probable carboxylesterase 1
AtCXE1
EC 3.1.1.1
Gene Name CXE1 At1g19190 T29M8.6
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MDSEIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGSDLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENARRVLRNFAEFLKEETF
Enzyme Length 318
Uniprot Accession Number Q9LMA7
Absorption
Active Site ACT_SITE 163; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038; ACT_SITE 258; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038; ACT_SITE 290; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
DNA Binding
EC Number 3.1.1.1
Enzyme Function FUNCTION: Carboxylesterase acting on esters with varying acyl chain length. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Modified residue (1); Motif (1)
Keywords Acetylation;Hydrolase;Reference proteome;Serine esterase
Interact With
Induction
Subcellular Location
Modified Residue MOD_RES 1; /note=N-acetylmethionine; /evidence=ECO:0000250|UniProtKB:Q9SMN0
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16553894;
Motif MOTIF 79..81; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3
Gene Encoded By
Mass 36,081
Kinetics
Metal Binding
Rhea ID RHEA:21164
Cross Reference Brenda