Detail Information for IndEnz0010001131
IED ID IndEnz0010001131
Enzyme Type ID esterase001131
Protein Name Probable carboxylesterase 18
AtCXE18
EC 3.1.1.1
Gene Name CXE18 At5g23530 MQM1.21
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MATDSQPNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRDLWFRLYTPHVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAMGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFHAFYIFPELPEAGQLIMRIKDFVDERVASLSA
Enzyme Length 335
Uniprot Accession Number Q9LT10
Absorption
Active Site ACT_SITE 173; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 274; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 304; /evidence=ECO:0000250|UniProtKB:Q5NUF3
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
DNA Binding
EC Number 3.1.1.1
Enzyme Function FUNCTION: Carboxylesterase acting on esters with varying acyl chain length. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Initiator methionine (1); Modified residue (1); Motif (1); Sequence conflict (1)
Keywords Acetylation;Hydrolase;Reference proteome;Serine esterase
Interact With
Induction
Subcellular Location
Modified Residue MOD_RES 2; /note=N-acetylalanine; /evidence=ECO:0007744|PubMed:22223895
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16581873; 16709201; 17270225; 18775970; 19714218;
Motif MOTIF 94..96; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3
Gene Encoded By
Mass 37,715
Kinetics
Metal Binding
Rhea ID RHEA:21164
Cross Reference Brenda