| IED ID | IndEnz0010001146 |
| Enzyme Type ID | esterase001146 |
| Protein Name |
4-O-methyl-glucuronoyl methylesterase EC 3.1.1.117 Glucuronoyl esterase GE |
| Gene Name | cip2 TRIREDRAFT_123940 |
| Organism | Hypocrea jecorina (strain QM6a) (Trichoderma reesei) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea jecorina (Trichoderma reesei) Hypocrea jecorina (strain QM6a) (Trichoderma reesei) |
| Enzyme Sequence | MASRFFALLLLAIPIQAQSPVWGQCGGIGWSGPTTCVGGATCVSYNPYYSQCIPSTQASSSIASTTLVTSFTTTTATRTSASTPPASSTGAGGATCSALPGSITLRSNAKLNDLFTMFNGDKVTTKDKFSCRQAEMSELIQRYELGTLPGRPSTLTASFSGNTLTINCGEAGKSISFTVTITYPSSGTAPYPAIIGYGGGSLPAPAGVAMINFNNDNIAAQVNTGSRGQGKFYDLYGSSHSAGAMTAWAWGVSRVIDALELVPGARIDTTKIGVTGCSRNGKGAMVAGAFEKRIVLTLPQESGAGGSACWRISDYLKSQGANIQTASEIIGEDPWFSTTFNSYVNQVPVLPFDHHSLAALIAPRGLFVIDNNIDWLGPQSCFGCMTAAHMAWQALGVSDHMGYSQIGAHAHCAFPSNQQSQLTAFVQKFLLGQSTNTAIFQSDFSANQSQWIDWTTPTLS |
| Enzyme Length | 460 |
| Uniprot Accession Number | G0RV93 |
| Absorption | |
| Active Site | ACT_SITE 278; /note=Nucleophile; /evidence=ECO:0000305|PubMed:21661060; ACT_SITE 411; /note=Proton donor/acceptor; /evidence=ECO:0000305|PubMed:21661060 |
| Activity Regulation | |
| Binding Site | BINDING 282; /note=Substrate; /evidence=ECO:0000250|UniProtKB:G2QJR6; BINDING 324; /note=Substrate; /evidence=ECO:0000250|UniProtKB:G2QJR6; BINDING 332; /note=Substrate; /evidence=ECO:0000250|UniProtKB:G2QJR6; BINDING 375; /note=Substrate; /evidence=ECO:0000250|UniProtKB:G2QJR6 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 4-O-methyl-alpha-D-glucuronosyl ester derivative + H2O = 4-O-methyl-alpha-D-glucuronate derivative + an alcohol + H(+); Xref=Rhea:RHEA:67452, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30879, ChEBI:CHEBI:171667, ChEBI:CHEBI:171668; EC=3.1.1.117; Evidence={ECO:0000269|PubMed:17678650};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67453; Evidence={ECO:0000305|PubMed:17678650}; |
| DNA Binding | |
| EC Number | 3.1.1.117 |
| Enzyme Function | FUNCTION: Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin. Does not hydrolyze substrates of other carbohydrate esterases such as acetylxylan esterase, acetyl esterase and feruloyl esterase. {ECO:0000269|PubMed:12788920, ECO:0000269|PubMed:17678650}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 40-60 degrees Celsius. {ECO:0000269|PubMed:17678650}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5. {ECO:0000269|PubMed:17678650}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (10); Binding site (4); Chain (1); Disulfide bond (3); Domain (1); Glycosylation (1); Helix (18); Modified residue (1); Motif (1); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Lignin degradation;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Serine esterase;Signal |
| Interact With | |
| Induction | INDUCTION: Induced in the presence of lactose or sophorose. {ECO:0000269|PubMed:12788920}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | MOD_RES 18; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:17678650 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000305|PubMed:17678650 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 3PIC; |
| Mapped Pubmed ID | - |
| Motif | MOTIF 276..281; /note=GXSYXG catalytic site motif; /evidence=ECO:0000305|PubMed:21661060 |
| Gene Encoded By | |
| Mass | 48,296 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.5 mM for 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside {ECO:0000269|PubMed:17678650}; Vmax=5.5 umol/min/mg enzyme toward 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside {ECO:0000269|PubMed:17678650}; |
| Metal Binding | |
| Rhea ID | RHEA:67452; RHEA:67453 |
| Cross Reference Brenda | 3.1.1.B11; |