IED ID | IndEnz0010001181 |
Enzyme Type ID | esterase001181 |
Protein Name |
Esterase citA EC 3.1.2.- Citrinin synthesis protein A |
Gene Name | citA mrl1 |
Organism | Monascus ruber (Mold) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Monascus Monascus ruber (Mold) |
Enzyme Sequence | MVQTNLEVVDDTLHLPRILCLHGGGSNAAIFQAQCRRLIAQLRSEFRFVFAQAPFLSDAEPNVMSVYSQWGPFRRWLRWCPDHPEIRPEDAIRAIDDCLEDVKRQDDAKGATGAWVGLLGFSQGAKMCASLLYRQQIRQELRGRSFAGSDYRFGVLLAGRAPLVSLDPDLDLNSSLPDVSQITDAKYHGPSQDVLRIPTVHVHGMRDPHVDLHRQLFEEFCAPESRRLVEWDGDHRVPLKYNDVSLVAYQIRELATQTGAP |
Enzyme Length | 261 |
Uniprot Accession Number | A0A161CKG1 |
Absorption | |
Active Site | ACT_SITE 122; /note=Charge relay system; /evidence=ECO:0000269|PubMed:29189834; ACT_SITE 207; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P38777; ACT_SITE 235; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P38777 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | 3.1.2.- |
Enzyme Function | FUNCTION: Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the mycotoxin citrinin, a hepato-nephrotoxic compound to humans due to inhibition of respiration complex III (Ref.1, PubMed:29189834). The pathway begins with the synthesis of a keto-aldehyde intermediate by the citrinin PKS (pksCT also named citS) from successive condensations of 4 malonyl-CoA units, presumably with a simple acetyl-CoA starter unit (Ref.1, PubMed:29189834). Release of the keto-aldehyde intermediate is consistent with the presence of the C-terminal reductive release domain (Ref.1). CitA collaborates with citS by catalyzing the hydrolysis of ACP-bound acyl intermediates to free the ACP from stalled intermediates (PubMed:29189834). CitB then catalyzes the oxidation of the C-12 methyl of the ketone intermediate to an alcohol intermediate which is further oxidized by the oxidoreductase citC to produce a bisaldehyde intermediate (Ref.1). The fourth catalytic step is catalyzed by the citD aldehyde dehydrogenase (Ref.1). The final transformation is the reduction of C-3 by citE to provide the chemically stable citrinin nucleus (Ref.1). CitE appears highly selective for its substrate as its presence in any context other than a full complement of citS and citA-D does not result in observable new compounds (Ref.1). {ECO:0000269|PubMed:29189834, ECO:0000269|Ref.1}. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|Ref.1}. |
nucleotide Binding | |
Features | Active site (3); Chain (1); Mutagenesis (1) |
Keywords | Hydrolase |
Interact With | |
Induction | INDUCTION: Expression is stimulated under green light conditions (PubMed:27998068). {ECO:0000269|PubMed:27998068}. |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 29,501 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |