Detail Information for IndEnz0010001183
IED ID IndEnz0010001183
Enzyme Type ID esterase001183
Protein Name Probable cutinase 2
EC 3.1.1.74
Cutin hydrolase 2
Gene Name An11g00110
Organism Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus niger Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Enzyme Sequence MKLPYFLLGLAGLAAASPMGLAERQLSDGNELRDGSCKPIIFIFARASTEPGLLGISTGPAVCNDLKMAKAGQVLCQGVGPAYTADLMSNALPDNTSPAAISESESLFKLAASKCPNSQILAGGYSQGTAVMDDSIKQLPDDVKDKIKGVVLFGYTRNAQEGGQIGNFPKDKVKIYCAMGDLVCDGTLIVTAAHFTYVMNTGEASQWLESKLSDTTTSSLTGSSSSDTSSSTSTGDSSSESSSAAGLGGLSGLTGLGSSTSGGFPSLASLF
Enzyme Length 271
Uniprot Accession Number A5ABE6
Absorption
Active Site ACT_SITE 126; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10109; ACT_SITE 181; /evidence=ECO:0000255|PROSITE-ProRule:PRU10109; ACT_SITE 194; /note=Proton donor/acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10109
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Cutin + H(2)O = cutin monomers.; EC=3.1.1.74; Evidence={ECO:0000255|PROSITE-ProRule:PRU10109};
DNA Binding
EC Number 3.1.1.74
Enzyme Function FUNCTION: Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants (By similarity). Degrades cutin, a macromolecule that forms the structure of the plant cuticle (By similarity). {ECO:0000250|UniProtKB:P00590}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (3); Region (1); Signal peptide (1); Site (2)
Keywords Disulfide bond;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P11373}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..16; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 27,628
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda