IED ID | IndEnz0010001192 |
Enzyme Type ID | esterase001192 |
Protein Name |
Acyl-CoA transferase FPSE_08120 EC 2.8.3.- Fusarium detoxification of benzoxazolinone cluster protein FPSE_08120 FDB cluster protein FPSE_08120 |
Gene Name | FPSE_08120 |
Organism | Fusarium pseudograminearum (strain CS3096) (Wheat and barley crown-rot fungus) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Nectriaceae Fusarium Fusarium sambucinum species complex Fusarium pseudograminearum (Wheat and barley crown-rot fungus) Fusarium pseudograminearum (strain CS3096) (Wheat and barley crown-rot fungus) |
Enzyme Sequence | MARLLFSGQRLRPSFLRSYIRANPSSTPSATRAAINYRYNSNNAALQSASSSQGALTGIKIIDLSRVLAGPFCTQILADYGAEVTKVEAVGKGDDTRHWIMAGEKASWNESSGPISNYFAAVNRNKRSITVNFKKAEGRQLILDLIKDADVVVENFKPGTMERLGLGYDVLKELNPRIIYAGLSGYGRTGPYRTRGGYDPIAAAEAGLLHVTGEKNGPPVRAGIGLVDMSTGLFLHGAILSALIARARDGTGQRVDASLFETQLSLLTNVGLAWLNLGIEAERWGCQHPSIAPYDAFKTRDRYLVCGATNDNQYAALCCLLGVEHLVTDPRFITNPLRVQHREELAALLGPIFASKTIDEWIALFEPSGLPFGPINNMEATFAHPQTAARDMVIDVPMDAACAGSIKVIGPAVKFGDSKTGLRTGPPRLGQHTVEILEEIGMDAEAIAKYKEDGII |
Enzyme Length | 456 |
Uniprot Accession Number | K3VD64 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | 2.8.3.- |
Enzyme Function | FUNCTION: Acyl-CoA transferase; part of the Fusarium detoxification of benzoxazolinone cluster involved in the degradation of benzoxazolinones produced by the host plant (PubMed:26296598, PubMed:25727347, PubMed:26828593). Maize, wheat, and rye produce the 2 benzoxazinone phytoanticipins 2,4-dihy-droxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) that, due to their inherent instability once released, spontaneously degrade to the more stable corresponding benzoxazolinones, 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), respectively (By similarity). The first step in the detoxification of benzoxazolinones involves the hydrolysis of the cyclic ester bond of benzoxazolinones by the gamma-lactamase FDB1 to aminophenols (PubMed:26296598). FDB1 is able to convert 2-benzoxazolinone (BOA) into 2-aminophenol (2-AP), as well as 6-methoxy-2-benzoxazolinone (MBOA) into 5-methoxy-2-aminophenol (2-AMP) (PubMed:26296598, PubMed:25727347). The N-malonyltransferase FDB2 then metabolizes aminophenols via N-malonylation to non-toxic malonamic acids (PubMed:26296598). FDB2 converts 2-AP into N-(2-hydroxyphenyl) malonamic acid (HPMA) and 2-AMP into N-(2-hydroxy-4-methoxyphenyl) malonamic acid (HMPMA) (PubMed:26296598). The cluster contains also 2 transcription factors (FDB3 and FPSE_08121), an aldo-keto reductase (FPSE_08125) that possibly associates with a ketone component of BOA and MBOA degradation, an esterase (FPSE_08126), an acyl-CoA transferase (FPSE_08120), a solute carrier protein (FPSE_08119) and a transmembrane transporter (FPSE_08127) proposed to shuttle metabolites of benzoxazolinone degradation (Probable). {ECO:0000250|UniProtKB:W7MLD5, ECO:0000269|PubMed:25727347, ECO:0000269|PubMed:26296598, ECO:0000269|PubMed:26828593, ECO:0000305|PubMed:26828593}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Chain (1); Transit peptide (1) |
Keywords | Acyltransferase;Mitochondrion;Transferase;Transit peptide |
Interact With | |
Induction | INDUCTION: Expression is induced in response to 2-benzoxasolinone (BOA) exposure (PubMed:25727347). Expression is also induced in response to 6-methoxy-2-benzoxazolinone (MBOA) and 2-aminophenol (2-AP) treatment (PubMed:26828593). {ECO:0000269|PubMed:25727347, ECO:0000269|PubMed:26828593}. |
Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 49,273 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |