| IED ID | IndEnz0010001201 |
| Enzyme Type ID | esterase001201 |
| Protein Name |
Fumonisin B1 esterase EC 3.1.1.87 |
| Gene Name | fumD |
| Organism | Sphingopyxis macrogoltabida (Sphingomonas macrogoltabidus) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingopyxis Sphingopyxis macrogoltabida (Sphingomonas macrogoltabidus) |
| Enzyme Sequence | MKEHQCRGGRASPAAPATWLARISVSRGASAIAWTFMLGATAIPVAAQTDDPKLVRHTQSGAVEGVEGDVETFLGIPFAAPPVGDLRWRPPAPPRAWAGTRDGRRFAPDCIGNERLREGSRAAGTSEDCLYLNIWSPKQVGKGGLPVMIWVYGGGFSGGSGAVPYYDGSALAQKGVVVVTFNYRAGILGFLAHPALSKESPNGVSGNYGLLDMLAAFKWVQNNIREFGGDPNRVTVFGESAGASALGLLLTSPLSESAFNQAILQSPGLARPLATLSESEANGLELGADISALRRADAGELTKIAQSRIPMSRQFTKPRPMGPILDGYVLRTLDVDAFAKGAFRKIPVLVGGNADEGRAFTDRLPVKTVLEYRAYLTEQFGDEADAWERCYPANSDADVPAAVARLFGDSQFNNGIELLSAAFAKWRTPLWRYRFTGIPGAGRRPATHGDEIPYVFANLGPSSVSMFGSLEGGAGASDIKLATEMSAAWVSFAVHGVPDQGTKSHWPRFERRGEIMTFGSQVGSGEGLGVSPSKACQPSK |
| Enzyme Length | 540 |
| Uniprot Accession Number | D2D3B6 |
| Absorption | |
| Active Site | ACT_SITE 240; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 356; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 448; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=fumonisin B1 + 2 H2O = (2S,3S,5R,10R,12S,14S,15R,16R)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + 2 H(+) + 2 tricarballylate; Xref=Rhea:RHEA:30363, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:62517, ChEBI:CHEBI:62526, ChEBI:CHEBI:62554; EC=3.1.1.87; Evidence={ECO:0000269|PubMed:19922747}; |
| DNA Binding | |
| EC Number | 3.1.1.87 |
| Enzyme Function | FUNCTION: Involved in degradation of fumonisin B1. Catalyzes the hydrolysis of fumonisin B1 (FB1) to aminopentol (HFB1). {ECO:0000269|PubMed:19922747}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Region (1) |
| Keywords | Hydrolase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 57,362 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:30363 |
| Cross Reference Brenda | 3.1.1.87; |