| IED ID | IndEnz0010001242 |
| Enzyme Type ID | esterase001242 |
| Protein Name |
N-acetylneuraminate 9-O-acetyltransferase EC 2.3.1.45 CAS1 domain-containing protein 1 Sialate O-acetyltransferase SOAT |
| Gene Name | casd1 si:dkey-104m9.2 zgc:136291 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MAVLAYNLGKREINQYFSIKNAKLLAAAAVVLLTVFHAASRHYGSSDTCDWLLSSGRFLGDNVWQPYGCMLHKYKSTEAKFCLREKRIAFVGDSRIRQLFYSFIKMMNPEVKEVGNKHENIPFVDGDSTVNFLWYAEVNNSLKEQLMLWTEGSASKPHVIIIGAATWSIKLHNGKSEALFQYKANLTAIADTLEKLAEHSEVYWVLQDPVYEDVLSESRKMITNEQINLYNEAAVSTLNTSKKKVKFLEASRQAAMETISQSVDGLHLPESTRDVGAMVLMNSMCNKILKPIDGSCCQSAPPLSVLQKLAAAVLLVSVVCFVLLGFSSHRKSRPAPDVESGEEKKHPAAVGQLNPKGPLLAIGKMSLIMLYFYLCDRADIFMKEQKFYTHSAFFIPLIYIFVLGVFYSENSKETKLLNREQTDEWKGWMQLVILIYHISGASAFIPVYMHVRVLVAAYLFQTGYGHFSFFWLKGDFGLYRVCQVLFRLNFLVVVLCLVMDRPYQFYYFVPLVTFWFAVIYATMALWPQILQKQANGSAFWNLALLLKLLGLLLFIGFFAYSQELFEGIFSVWPLSKLFELQGSIHEWWFRWKLDRFAVVNGMLFAFIYLLLQKYQLLSEGKGEPLFSNKISNCLLFVSVVSFMTYSIWASGCKNKSECNEMHPYISVILAFILIRNIPGYARSLYSSFFAWFGKISLELFICQYHIWLAADTKGILVLIPGNPTLNIIVSTFIFVCVAHEISQITNDLAQVAIPKESGPLLKRLLGAGVFLVLVLTLSQKD |
| Enzyme Length | 781 |
| Uniprot Accession Number | Q1LW89 |
| Absorption | |
| Active Site | ACT_SITE 94; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q96PB1; ACT_SITE 264; /evidence=ECO:0000250|UniProtKB:Q96PB1; ACT_SITE 267; /evidence=ECO:0000250|UniProtKB:Q96PB1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetyl-CoA + N-acetylneuraminate = CoA + N-acetyl-9-O-acetylneuraminate; Xref=Rhea:RHEA:26043, ChEBI:CHEBI:28999, ChEBI:CHEBI:35418, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.45; Evidence={ECO:0000250|UniProtKB:Q96PB1}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + N-acetylneuraminate = CoA + N-acetyl-7-O-acetylneuraminate; Xref=Rhea:RHEA:20808, ChEBI:CHEBI:28944, ChEBI:CHEBI:35418, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.45; Evidence={ECO:0000250|UniProtKB:Q96PB1}; |
| DNA Binding | |
| EC Number | 2.3.1.45 |
| Enzyme Function | FUNCTION: O-acetyltransferase that catalyzes 9-O-acetylation of sialic acids. Sialic acids are sugars at the reducing end of glycoproteins and glycolipids, and are involved in various processes such as cell-cell interactions, host-pathogen recognition. {ECO:0000250|UniProtKB:Q96PB1}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Erroneous gene model prediction (1); Glycosylation (4); Region (1); Sequence caution (2); Topological domain (16); Transmembrane (15) |
| Keywords | Acyltransferase;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q96PB1}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q96PB1}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 30135188; |
| Motif | |
| Gene Encoded By | |
| Mass | 88,736 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:26043; RHEA:20808 |
| Cross Reference Brenda |