Detail Information for IndEnz0010001244
IED ID IndEnz0010001244
Enzyme Type ID esterase001244
Protein Name Ethanol acetyltransferase 2
EC 2.3.1.268
Acetyl-CoA hydrolase
EC 3.1.2.1
Acetyl-CoA thioesterase
Alcohol acetyltransferase
AAT
Ethyl acetate esterase
EC 3.1.1.-
Gene Name EAT2 AWRI3580_g2626
Organism Hanseniaspora uvarum (Yeast) (Kloeckera apiculata)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycodaceae Hanseniaspora Hanseniaspora uvarum (Yeast) (Kloeckera apiculata)
Enzyme Sequence MIFNSLSIKRLSSTXTSLPFKKHAKLNFDLYSPQHSTYGKLPYHCQEPIIFLHGIYGYSKSFNSDYQQLSNLLHTPIYSVDMRCHGETENCLPFTYDALAGDLDNFVITHNIKKPSLIGFSLGAKLAMLAILKSPHLYTSGVIVDNVPLKQPRIKPNLTAFGNALRDSVFKSGVKRNDPSWISKSFNVMKDVCSDMPANFYLLHNIQPKPSYLKKYSTEESENSLFCKVPIRELSSHVVENVPDWPEEDLAGVKTDVPILVVKASTSGFVNEDGVAALXKHFSDFTIVEVAGTHLVMKERPQEYISAVGRWFYQQNCKKAAALTKAKKTNDQKITQQPILSYKKIEIA
Enzyme Length 348
Uniprot Accession Number A0A1E5RIW9
Absorption
Active Site ACT_SITE 121; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6; ACT_SITE 145; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6; ACT_SITE 294; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=acetyl-CoA + ethanol = CoA + ethyl acetate; Xref=Rhea:RHEA:55972, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.268; Evidence={ECO:0000269|PubMed:28356220}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1; Evidence={ECO:0000250|UniProtKB:A0A1E3P8S6}; CATALYTIC ACTIVITY: Reaction=ethyl acetate + H2O = acetate + ethanol + H(+); Xref=Rhea:RHEA:58148, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:30089; Evidence={ECO:0000250|UniProtKB:A0A1E3P8S6};
DNA Binding
EC Number 2.3.1.268; 3.1.2.1; 3.1.1.-
Enzyme Function FUNCTION: Alcohol acetyltransferase that catalyzes the synthesis of ethyl acetate from ethanol and acetyl-CoA (PubMed:28356220). Can also function as a thioesterase by hydrolyzing acetyl-CoA in the absence of ethanol, as well as esterase hydrolyzing ethyl acetate (By similarity). {ECO:0000250|UniProtKB:A0A1E3P8S6, ECO:0000269|PubMed:28356220}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Domain (1); Sequence uncertainty (1); Transit peptide (1)
Keywords Hydrolase;Mitochondrion;Reference proteome;Transferase;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:A0A1E3P8S6}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 39,163
Kinetics
Metal Binding
Rhea ID RHEA:55972; RHEA:20289; RHEA:58148
Cross Reference Brenda