Detail Information for IndEnz0010001256
IED ID IndEnz0010001256
Enzyme Type ID esterase001256
Protein Name Acyl-protein thioesterase 1
EC 3.1.2.-
Palmitoyl-protein hydrolase
EC 3.1.2.22
Gene Name UMAG_00130
Organism Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Ustilaginomycotina Ustilaginomycetes Ustilaginales Ustilaginaceae Ustilago Ustilago maydis (Corn smut fungus) Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Enzyme Sequence MSVLKTLVINPRSGVKPTATLFFLHGLGDSSAGWSDVAQMLSQRPSLSHVRFVLPNAPIQPVTLNMGMPMPSWFDILALDDLSGAEDEAGLLKSTDEIKKLIKAENDGTAKDLDGHKIPSERIVVGGFSQGGAISLLTGLTNPTPVAGVAALSTWLPLRAKIATLRTPTSKTLKVFQAHGDADPVVKYEYGQRTVDFLKNELALNDKDVEFHTYPRMPHSACPEEIRDLAAFLEKVIPAQ
Enzyme Length 240
Uniprot Accession Number Q4PID3
Absorption
Active Site ACT_SITE 129; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 183; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 219; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=H2O + S-hexadecanoyl-L-cysteinyl-[protein] = H(+) + hexadecanoate + L-cysteinyl-[protein]; Xref=Rhea:RHEA:19233, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:74151; EC=3.1.2.22; Evidence={ECO:0000250|UniProtKB:Q12354};
DNA Binding
EC Number 3.1.2.-; 3.1.2.22
Enzyme Function FUNCTION: Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo. {ECO:0000250|UniProtKB:Q12354}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1)
Keywords Cytoplasm;Fatty acid metabolism;Hydrolase;Lipid metabolism;Nucleus;Reference proteome;Serine esterase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q12354}. Nucleus {ECO:0000250|UniProtKB:Q12354}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 25,981
Kinetics
Metal Binding
Rhea ID RHEA:19233
Cross Reference Brenda