| IED ID | IndEnz0010001271 |
| Enzyme Type ID | esterase001271 |
| Protein Name |
Teichoic acid D-alanine hydrolase EC 3.1.1.103 Teichoic acid D-alanine esterase |
| Gene Name | fmtA fmt |
| Organism | Staphylococcus aureus |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus aureus |
| Enzyme Sequence | MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETKLSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKMSKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFKTSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLVLAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRPNILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFYAKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTKGVIVQ |
| Enzyme Length | 397 |
| Uniprot Accession Number | O50608 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=((4-D-Ala)-(2-GlcNAc)-Rib-ol-P)(n)-(Gro-P)(m)-beta-D-ManNAc-(1->4)-alpha-D-GlcNAc-P-peptidoglycan + n H(2)O = ((2-GlcNAc)-Rib-ol-P)(n)-(Gro-P)(m)-beta-D-ManNAc-(1->4)-alpha-D-GlcNAc-P-peptidoglycan + n D-alanine.; EC=3.1.1.103; Evidence={ECO:0000250|UniProtKB:Q7A2T0}; |
| DNA Binding | |
| EC Number | 3.1.1.103 |
| Enzyme Function | FUNCTION: Catalyzes the liberation of D-alanyl moieties present on wall teichoic acid (WTA) and lipoteichoic acid (LTA) (By similarity). Affects the methicillin resistance level and autolysis in the presence of Triton X-100 as well as the cell wall structure (PubMed:9371333, PubMed:10471551). {ECO:0000250|UniProtKB:Q7A2T0, ECO:0000269|PubMed:10471551, ECO:0000269|PubMed:9371333}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (13); Chain (1); Helix (15); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Antibiotic resistance;Cell membrane;Cell wall biogenesis/degradation;Hydrolase;Membrane;Signal |
| Interact With | |
| Induction | INDUCTION: Transcription is dose dependently increased by the addition of beta-lactam antibiotics, fosfomycin, and bacitracin. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 5ZH8; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 46,067 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.1.1.103; |