| IED ID | IndEnz0010001286 |
| Enzyme Type ID | esterase001286 |
| Protein Name |
Ethanol acetyltransferase 1 EC 2.3.1.268 Acetyl-CoA hydrolase EC 3.1.2.1 Acetyl-CoA thioesterase Alcohol acetyltransferase AAT Ethyl acetate esterase EC 3.1.1.- |
| Gene Name | EAT1 BN1211_6162 CYBJADRAFT_189656 |
| Organism | Cyberlindnera jadinii (strain ATCC 18201 / CBS 1600 / BCRC 20928 / JCM 3617 / NBRC 0987 / NRRL Y-1542) (Torula yeast) (Candida utilis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Phaffomycetaceae Cyberlindnera Cyberlindnera jadinii (Torula yeast) (Pichia jadinii) Cyberlindnera jadinii (strain ATCC 18201 / CBS 1600 / BCRC 20928 / JCM 3617 / NBRC 0987 / NRRL Y-1542) (Torula yeast) (Candida utilis) |
| Enzyme Sequence | MFPTRVLRSTLQKLPHRETVPMAYDLHMPQRSLFQNLNEKHTEPIVFLHGIFGSKKSYELDSKMIAHGTHTPVYTVDIRNHGQTSHAMPFNYDTLAQDVKEFCHVQGLKSVKLVGYSLGAKISMLAALKYPELVKSAVIIDNAPVKQPYIELYMKQYVKSMLHVLEEAKIKTTDKDWKNKASDAMKKFLPNGVIRKNLLVNLVNKKPDGFESPVVNFDEGLIQFLNPIRQMEEAAVKDVTDWPEESTEGLKYNGPVKFIKGLKSPFINEEGMTKIQDIFPNNEFANVNSSHDILDQRPTEYVKIICDFFNAHRYESAPSNTIIGHKDFKTTIDMRV |
| Enzyme Length | 336 |
| Uniprot Accession Number | A0A1E4S2P1 |
| Absorption | |
| Active Site | ACT_SITE 117; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6; ACT_SITE 141; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6; ACT_SITE 291; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetyl-CoA + ethanol = CoA + ethyl acetate; Xref=Rhea:RHEA:55972, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.268; Evidence={ECO:0000269|PubMed:28356220}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1; Evidence={ECO:0000250|UniProtKB:A0A1E3P8S6}; CATALYTIC ACTIVITY: Reaction=ethyl acetate + H2O = acetate + ethanol + H(+); Xref=Rhea:RHEA:58148, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:30089; Evidence={ECO:0000250|UniProtKB:A0A1E3P8S6}; |
| DNA Binding | |
| EC Number | 2.3.1.268; 3.1.2.1; 3.1.1.- |
| Enzyme Function | FUNCTION: Alcohol acetyltransferase that catalyzes the synthesis of ethyl acetate from ethanol and acetyl-CoA (PubMed:28356220). Can also function as a thioesterase by hydrolyzing acetyl-CoA in the absence of ethanol, as well as esterase hydrolyzing ethyl acetate (By similarity). {ECO:0000250|UniProtKB:A0A1E3P8S6, ECO:0000269|PubMed:28356220}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Transit peptide (1) |
| Keywords | Hydrolase;Mitochondrion;Reference proteome;Transferase;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:A0A1E3P8S6}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,427 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:55972; RHEA:20289; RHEA:58148 |
| Cross Reference Brenda |