Detail Information for IndEnz0010001307
IED ID IndEnz0010001307
Enzyme Type ID esterase001307
Protein Name Ethanol acetyltransferase 1
EC 2.3.1.268
Acetyl-CoA hydrolase
EC 3.1.2.1
Acetyl-CoA thioesterase
Alcohol acetyltransferase
AAT
Ethyl acetate esterase
EC 3.1.1.-
Gene Name EAT1 AWRI3580_g1230
Organism Hanseniaspora uvarum (Yeast) (Kloeckera apiculata)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycodaceae Hanseniaspora Hanseniaspora uvarum (Yeast) (Kloeckera apiculata)
Enzyme Sequence MFASNVVVLNKRSIRFIQTQLPVKATVDMKFDLHLPKRSVIGKLPYHVEEPIVFVHGLFGSKKNYFNDCEKLSNLLQTPIYTIDFRNHGETEHAMPFDYETLTNDLIHFVEKHNLKNPSLIGYSLGAKVSMLALLKQPSLFKSAMIIDNSPVVQPEIVPFLKVFRKACVETVNKAGIRADDKDYRAKANQYMSKFIPNAGIKGYLLQNCINKPPKNPSPVINYNDGMIHWKNPVNHMANVSEENVADWPTIPEGIKFNGPVGFLRGTKSDFVLQKGRDAIAKLFPNNRIFDINATHFILNERPSEYVGIVADWFKTGRHDVEKLAAKKAKNAQL
Enzyme Length 334
Uniprot Accession Number A0A1E5RUL9
Absorption
Active Site ACT_SITE 124; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6; ACT_SITE 148; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6; ACT_SITE 296; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:A0A1E3P8S6
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=acetyl-CoA + ethanol = CoA + ethyl acetate; Xref=Rhea:RHEA:55972, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.268; Evidence={ECO:0000269|PubMed:28356220}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1; Evidence={ECO:0000250|UniProtKB:A0A1E3P8S6}; CATALYTIC ACTIVITY: Reaction=ethyl acetate + H2O = acetate + ethanol + H(+); Xref=Rhea:RHEA:58148, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:30089; Evidence={ECO:0000250|UniProtKB:A0A1E3P8S6};
DNA Binding
EC Number 2.3.1.268; 3.1.2.1; 3.1.1.-
Enzyme Function FUNCTION: Alcohol acetyltransferase that catalyzes the synthesis of ethyl acetate from ethanol and acetyl-CoA (PubMed:28356220). Can also function as a thioesterase by hydrolyzing acetyl-CoA in the absence of ethanol, as well as esterase hydrolyzing ethyl acetate (By similarity). {ECO:0000250|UniProtKB:A0A1E3P8S6, ECO:0000269|PubMed:28356220}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Transit peptide (1)
Keywords Hydrolase;Mitochondrion;Reference proteome;Transferase;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:A0A1E3P8S6}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 37,862
Kinetics
Metal Binding
Rhea ID RHEA:55972; RHEA:20289; RHEA:58148
Cross Reference Brenda