Detail Information for IndEnz0010001318
IED ID IndEnz0010001318
Enzyme Type ID esterase001318
Protein Name Serine protease Hip1
EC 3.4.21.-
Carboxylesterase A
Gene Name hip1 caeA MT2282
Organism Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Enzyme Sequence MGMRLSRRDKIARMLLIWAALAAVALVLVGCIRVVGGRARMAEPKLGQPVEWTPCRSSNPQVKIPGGALCGKLAVPVDYDRPDGDVAALALIRFPATGDKIGSLVINPGGPGESGIEAALGVFQTLPKRVHERFDLVGFDPRGVASSRPAIWCNSDADNDRLRAEPQVDYSREGVAHIENETKQFVGRCVDKMGKNFLAHVGTVNVAKDLDAIRAALGDDKLTYLGYSYGTRIGSAYAEEFPQRVRAMILDGAVDPNADPIEAELRQAKGFQDAFNNYAADCAKNAGCPLGADPAKAVEVYHSLVDPLVDPDNPRISRPARTKDPRGLSYSDAIVGTIMALYSPNLWQHLTDGLSELVDNRGDTLLALADMYMRRDSHGRYNNSGDARVAINCVDQPPVTDRDKVIDEDRRAREIAPFMSYGKFTGDAPLGTCAFWPVPPTSQPHAVSAPGLVPTVVVSTTHDPATPYKAGVDLANQLRGSLLTFDGTQHTVVFQGDSCIDEYVTAYLIGGTTPPSGAKC
Enzyme Length 520
Uniprot Accession Number P9WHR2
Absorption
Active Site ACT_SITE 228; /note=Nucleophile; /evidence=ECO:0000305|PubMed:17428787; ACT_SITE 463; /evidence=ECO:0000305|PubMed:17428787; ACT_SITE 490; /note=Proton donor; /evidence=ECO:0000305|PubMed:17428787
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.21.-
Enzyme Function FUNCTION: Serine protease that promotes tuberculosis (TB) pathogenesis by promoting the processing and the extracellular release of the M.tuberculosis (Mtb) heat-shock protein GroEL2 (By similarity). In vitro, catalyzes the cleavage of ester bonds. Esterase activity increases with increasing carbon chain length of the substrate (PubMed:17428787). {ECO:0000250|UniProtKB:P9WHR3, ECO:0000269|PubMed:17428787}.; FUNCTION: Key immunomodulatory virulence factor, which promotes survival in host macrophages and modulates host immune responses. {ECO:0000250|UniProtKB:P9WHR3}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7. {ECO:0000269|PubMed:17428787};
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Domain (1); Erroneous initiation (1); Lipidation (2); Mutagenesis (3); Signal peptide (1)
Keywords Cell membrane;Hydrolase;Lipoprotein;Membrane;Palmitate;Signal;Virulence
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell envelope {ECO:0000269|PubMed:17428787}. Cell membrane {ECO:0000255|PROSITE-ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..30; /evidence=ECO:0000255|PROSITE-ProRule:PRU00303
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 55,924
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=738 uM for 4-methylumbelliferyl butyrate {ECO:0000269|PubMed:17428787}; Vmax=128 umol/min/mg enzyme with 4-methylumbelliferyl butyrate as substrate {ECO:0000269|PubMed:17428787};
Metal Binding
Rhea ID
Cross Reference Brenda