Detail Information for IndEnz0010001325
IED ID IndEnz0010001325
Enzyme Type ID esterase001325
Protein Name Gut esterase 1
EC 3.1.1.1
Non-specific carboxylesterase
Gene Name ges-1 R12A1.4
Organism Caenorhabditis elegans
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans
Enzyme Sequence MRIFLVSVILINACWAGPIVETNYGKVEGIEYEGAEVFLAIPFAKPPVDDLRFEKPVAPDPWEDVYPATQYRNDCTPHYRLVAQFSSYSGEDCLTLNIIKPKKAEKLPVLFWIHGGGYEIGSASQHGYEFFAKRYASQGVIVATVQYRLGFMGFFSEGTSDVQGNWGLFDQAAALEFVKSNIENFGGDPNQITIWGYSAGAASVSQLTMSPYTRDSYSKAIIMSASSFVGWATGPNVVETSKQLAEILGCPWPGAKECMKKKSLHEIFDAIEVQGWTTGTIDILRWSPVIDGDFMTKNPEELIKESPVKPTLIGMSNKEGSYFAALNMGRVIADFGLSPEDMPKVDEEFISEIIGRKLLYNNRYGENREKVWNDILDFYVKQGKPAEVKDLNGFYVDRYSELLSDITFNVPILREITSRVERKTPVWTYRMDHYDKNIWKKHIPEQARGSPHANEYHYLFDMPVMAKIDMKKEPDSWIQNDLIDMVISFAKTGVPQIEDVEWRPVSDPDDVNFLNIRSDGVSIEHGLFQEPLAFWNELRQREGFDLIDPTNSAMHSSNKDEL
Enzyme Length 562
Uniprot Accession Number Q04457
Absorption
Active Site ACT_SITE 198; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 319; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 452; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
DNA Binding
EC Number 3.1.1.1
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (2); Glycosylation (1); Motif (1); Signal peptide (1)
Keywords Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Reference proteome;Serine esterase;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Endoplasmic reticulum lumen.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..16; /evidence=ECO:0000269|PubMed:8445654
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11381264; 11784040; 12845331; 16777607; 18006689; 21177967; 21367940; 22286215; 22508763; 22560298; 2379823; 23800452; 25487147; 26009280; 29309033; 7649372; 8395047;
Motif MOTIF 559..562; /note=Prevents secretion from ER
Gene Encoded By
Mass 63,722
Kinetics
Metal Binding
Rhea ID RHEA:21164
Cross Reference Brenda