| IED ID | IndEnz0010001361 |
| Enzyme Type ID | esterase001361 |
| Protein Name |
Mono 2-hydroxyethyl terephthalate hydrolase MHET hydrolase MHETase EC 3.1.1.102 |
| Gene Name | ISF6_0224 |
| Organism | Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiales genera incertae sedis Ideonella Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6) |
| Enzyme Sequence | MQTTVTTMLLASVALAACAGGGSTPLPLPQQQPPQQEPPPPPVPLASRAACEALKDGNGDMVWPNAATVVEVAAWRDAAPATASAAALPEHCEVSGAIAKRTGIDGYPYEIKFRLRMPAEWNGRFFMEGGSGTNGSLSAATGSIGGGQIASALSRNFATIATDGGHDNAVNDNPDALGTVAFGLDPQARLDMGYNSYDQVTQAGKAAVARFYGRAADKSYFIGCSEGGREGMMLSQRFPSHYDGIVAGAPGYQLPKAGISGAWTTQSLAPAAVGLDAQGVPLINKSFSDADLHLLSQAILGTCDALDGLADGIVDNYRACQAAFDPATAANPANGQALQCVGAKTADCLSPVQVTAIKRAMAGPVNSAGTPLYNRWAWDAGMSGLSGTTYNQGWRSWWLGSFNSSANNAQRVSGFSARSWLVDFATPPEPMPMTQVAARMMKFDFDIDPLKIWATSGQFTQSSMDWHGATSTDLAAFRDRGGKMILYHGMSDAAFSALDTADYYERLGAAMPGAAGFARLFLVPGMNHCSGGPGTDRFDMLTPLVAWVERGEAPDQISAWSGTPGYFGVAARTRPLCPYPQIARYKGSGDINTEANFACAAPP |
| Enzyme Length | 603 |
| Uniprot Accession Number | A0A0K8P8E7 |
| Absorption | |
| Active Site | ACT_SITE 225; /note=Acyl-ester intermediate; /evidence=ECO:0000305|PubMed:30979881; ACT_SITE 492; /note=Charge relay system; /evidence=ECO:0000305|PubMed:30979881; ACT_SITE 528; /note=Charge relay system; /evidence=ECO:0000305|PubMed:30979881 |
| Activity Regulation | |
| Binding Site | BINDING 132; /note=4-[(2-hydroxyethoxy)carbonyl]benzoate; via amide nitrogen; /evidence=ECO:0000305|PubMed:30979881; BINDING 226; /note=4-[(2-hydroxyethoxy)carbonyl]benzoate; via amide nitrogen; /evidence=ECO:0000305|PubMed:30979881; BINDING 411; /note=4-[(2-hydroxyethoxy)carbonyl]benzoate; /evidence=ECO:0000305|PubMed:30979881; BINDING 416; /note=4-[(2-hydroxyethoxy)carbonyl]benzoate; /evidence=ECO:0000305|PubMed:30979881; BINDING 528; /note=4-[(2-hydroxyethoxy)carbonyl]benzoate; /evidence=ECO:0000305|PubMed:30979881 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=4-[(2-hydroxyethoxy)carbonyl]benzoate + H2O = ethylene glycol + H(+) + terephthalate; Xref=Rhea:RHEA:49532, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30043, ChEBI:CHEBI:30742, ChEBI:CHEBI:131704; EC=3.1.1.102; Evidence={ECO:0000269|PubMed:26965627, ECO:0000269|PubMed:30979881};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:49533; Evidence={ECO:0000269|PubMed:26965627}; |
| DNA Binding | |
| EC Number | 3.1.1.102 |
| Enzyme Function | FUNCTION: Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Likely acts synergistically with PETase to depolymerize PET. Catalyzes the hydrolysis of mono(2-hydroxyethyl) terephthalate (MHET) into its two environmentally benign monomers, terephthalate and ethylene glycol. Does not show activity against PET, bis(hydroxyethyl) terephthalate (BHET), pNP-aliphatic esters or typical aromatic ester compounds catalyzed by the tannase family enzymes, such as ethyl gallate and ethyl ferulate. {ECO:0000269|PubMed:26965627}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (22); Binding site (5); Chain (1); Compositional bias (1); Disulfide bond (5); Helix (27); Lipidation (2); Metal binding (5); Mutagenesis (6); Region (1); Signal peptide (1); Turn (8) |
| Keywords | 3D-structure;Calcium;Cell outer membrane;Disulfide bond;Hydrolase;Lipoprotein;Membrane;Metal-binding;Palmitate;Reference proteome;Serine esterase;Signal |
| Interact With | |
| Induction | INDUCTION: Highly up-regulated during growth on PET film. {ECO:0000269|PubMed:26965627}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305|PubMed:26965627}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303, ECO:0000305|PubMed:26965627}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255|PROSITE-ProRule:PRU00303 |
| Structure 3D | X-ray crystallography (9) |
| Cross Reference PDB | 6JTT; 6JTU; 6QG9; 6QGA; 6QGB; 6QZ1; 6QZ2; 6QZ3; 6QZ4; |
| Mapped Pubmed ID | 32989159; |
| Motif | |
| Gene Encoded By | |
| Mass | 63,103 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=7.3 uM for mono(2-hydroxyethyl) terephthalate (at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:26965627}; Note=kcat is 31 sec(-1) for the hydrolysis of mono(2-hydroxyethyl) terephthalate (at pH 7 and 30 degrees Celsius). {ECO:0000269|PubMed:26965627}; |
| Metal Binding | METAL 304; /note=Calcium; /evidence=ECO:0000269|PubMed:30979881; METAL 307; /note=Calcium; /evidence=ECO:0000269|PubMed:30979881; METAL 309; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000269|PubMed:30979881; METAL 311; /note=Calcium; /evidence=ECO:0000269|PubMed:30979881; METAL 313; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000269|PubMed:30979881 |
| Rhea ID | RHEA:49532; RHEA:49533 |
| Cross Reference Brenda | 3.1.1.102; |