IED ID | IndEnz0010001365 |
Enzyme Type ID | esterase001365 |
Protein Name |
Carboxylesterase 1 AeCXE1 EC 3.1.1.1 |
Gene Name | CXE1 |
Organism | Actinidia eriantha (Velvet vine) (Actinidia fulvicoma var. lanata) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids Ericales Actinidiaceae Actinidia Actinidia eriantha (Velvet vine) (Actinidia fulvicoma var. lanata) |
Enzyme Sequence | MSNDHLETTGSSDPNTNLLKYLPIVLNPDRTITRPIQIPSTAASPDPTSSSPVLTKDLALNPLHNTFVRLFLPRHALYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMAVHAGVVIASVDYRLAPEHRLPAAYDDAMEALQWIKDSRDEWLTNFADFSNCFIMGESAGGNIAYHAGLRAAAVADELLPLKIKGLVLDEPGFGGSKRTGSELRLANDSRLPTFVLDLIWELSLPMGADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGDPMIDRQMELAERLEKKGVDVVAQFDVGGYHAVKLEDPEKAKQFFVILKKFVVDSCTTKL |
Enzyme Length | 335 |
Uniprot Accession Number | Q0ZPV7 |
Absorption | |
Active Site | ACT_SITE 169; /evidence=ECO:0000305; ACT_SITE 276; /evidence=ECO:0000305; ACT_SITE 306; /evidence=ECO:0000305 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; |
DNA Binding | |
EC Number | 3.1.1.1 |
Enzyme Function | FUNCTION: Carboxylesterase acting on esters with varying acyl chain length. {ECO:0000269|PubMed:17256879}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Beta strand (9); Chain (1); Helix (12); Motif (1); Region (2); Turn (6) |
Keywords | 3D-structure;Hydrolase;Serine esterase |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | X-ray crystallography (2) |
Cross Reference PDB | 2O7R; 2O7V; |
Mapped Pubmed ID | - |
Motif | MOTIF 90..92; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
Gene Encoded By | |
Mass | 37,052 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=33.3 uM for 4-methylumbelliferyl acetate {ECO:0000269|PubMed:17256879}; KM=16.7 uM for 4-methylumbelliferyl butyrate {ECO:0000269|PubMed:17256879}; KM=19.1 uM for 4-methylumbelliferyl heptanoate {ECO:0000269|PubMed:17256879}; KM=6.34 uM for 4-methylumbelliferyl octanoate {ECO:0000269|PubMed:17256879}; KM=0.117 uM for 4-methylumbelliferyl laureate {ECO:0000269|PubMed:17256879}; KM=0.024 uM for 4-methylumbelliferyl palmitate {ECO:0000269|PubMed:17256879}; |
Metal Binding | |
Rhea ID | RHEA:21164 |
Cross Reference Brenda | 3.1.1.1; |