Detail Information for IndEnz0010001376
IED ID IndEnz0010001376
Enzyme Type ID esterase001376
Protein Name Palmitoleoyl-protein carboxylesterase NOTUM
EC 3.1.1.98
Gene Name Notum
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MGGEVRVLLLLGLLHWVGGSEGRKTWRRRGQQPPQPPPPPPLPQRAEVEPGAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSSAGGTGVLLNVDRVAELLEELGYPSIQVRGLADSGWFLDNKQYRRSDCIDTINCAPTDAIRRGIRYWSGMVPERCQRQFKEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGQWLYIQNLGRELRGTLKDVQASFAPACLSHEIIIRSYWTDVQVKGTSLPRALHCWDRSFHDSHKASKTPMKGCPFHLVDSCPWPHCNPSCPTIRDQFTGQEMNVAQFLMHMGFDVQTVAQQQGMEPSKLLGMLSNGN
Enzyme Length 503
Uniprot Accession Number Q8R116
Absorption
Active Site ACT_SITE 239; /note=Charge relay system; /evidence=ECO:0000305|PubMed:25771893; ACT_SITE 347; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 396; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[Wnt protein]-O-(9Z)-hexadecenoyl-L-serine + H2O = (9Z)-hexadecenoate + [Wnt protein]-L-serine + H(+); Xref=Rhea:RHEA:45340, Rhea:RHEA-COMP:11170, Rhea:RHEA-COMP:11171, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:32372, ChEBI:CHEBI:85189; EC=3.1.1.98; Evidence={ECO:0000250|UniProtKB:Q6P988};
DNA Binding
EC Number 3.1.1.98
Enzyme Function FUNCTION: Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors. {ECO:0000269|PubMed:25771893}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Alternative sequence (2); Chain (1); Compositional bias (1); Glycosylation (1); Modified residue (1); Mutagenesis (1); Region (1); Sequence conflict (1); Signal peptide (1)
Keywords Alternative splicing;Glycoprotein;Hydrolase;Phosphoprotein;Reference proteome;Secreted;Serine esterase;Signal;Wnt signaling pathway
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VUX3}.
Modified Residue MOD_RES 88; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q6P988
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11217851; 12466851; 14610273; 18429952; 18799693; 21267068; 21677750; 24475213; 25645944; 26393505; 26517809; 26926082; 26974334; 29428562; 30571485; 30622831; 31292548; 31307226; 32541007; 32579931; 33328171; 33647468; 33749332; 34079124; 34223673; 34385484; 34851738;
Motif
Gene Encoded By
Mass 56,584
Kinetics
Metal Binding
Rhea ID RHEA:45340
Cross Reference Brenda