| IED ID | IndEnz0010001377 |
| Enzyme Type ID | esterase001377 |
| Protein Name |
Lysophospholipase nte1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | nte1 An14g03050 |
| Organism | Aspergillus niger (strain CBS 513.88 / FGSC A1513) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus niger Aspergillus niger (strain CBS 513.88 / FGSC A1513) |
| Enzyme Sequence | MATGDGIIAAPPSLESSSLDPLHVLPASSSTAARSLATSIPALTASFSVVSGFSSHLPPPPVTPPAPSTMVGWIGWIFSFIFQVIPSVLYWIVTFTTITLPTWLFTLFSMSLTFTMNFTTLLLIALAVVSTISWFIRYRFLNMYSRLPPEPQRKEPQLDLFPDVPDSDTKPGLANYLDEFLSAIKVFGYLERPVFHELTRTMQTRKLIAGETLLLEEEKGFCLVVDGLVQIFVKSMRDRKSGSDEELNRMAGESSDEDDHRPDGRQGYQLLTEVKNGASMSSLFSILSLFTEDVQLRYADSSASSASSIGPGLAIGPDSFPASPREMDDSPHVYQGDRLDPASHRNSTAEDLPAVPPLNLGESQVPPAHSARSESRKYSGKTRRKSVHPDIVARAMVDTTIAIIPASAFRRLTRVYPKATAHIVQVILTRLQRVTFATAHSYLGLNNEVLGIEKQMTKFTTYDLPNELRGSALDRLKDKFIKERDRLGQEEVTKGIALHNPYGGRRPRSSSFRRKEAALQAKMVASKRPVSMVAQDSALSDRENSGVSPGDLLSTIQLSRFGPRYEHLAPRLLSPLTEKEHSPLRSPSPMIPGRASPFHRKESLDEDALFRESILECIMKGIGLTGSTNDFLRKSSHPSGDVSPKLLSYDSRRQKAVFSNNAFGFIDPYEGSADGESESMMSMSVTSAGGTSPIVNLREELRNDIEIVYFPQGSVLVEQGERHPGLYYVVDGFLDVGIPVDDKEEDLVGSSRPAHEELFPMLRRTNTSSSRVSGSAAAANDPRRKKQSRRSLYLIKPGGIQGYVGAVASYRSYTDVVAKTDVYVGFLPRASLERIAERYPLALLTLAKRLTSLLPRLLLHIDFALEWVQVSAGQVIYHQGDESDAIYLALNGRLRSVHEGPNGKMTVVGEYGQGESVGELEVMTESTRPATLHAIRDTELAKFPRTLFNSLAQEHPGITIQVSKLIAQRMRDLVETPLAEKGGEPGVSGTVKTAKSTLNLRTVGILPVTAGVPVVEFGNRLLQALHQIGVTNGATSLNQAAILNHLGRHAFSKMGKLKLSQYLADLEEKYGMVLYIADTSVNSPWTQTCITQADCILLVGLAESTPSIGEYERFLLGMKTTARKELVLLHGERYCPPGLTRQWLKNRVWINGGHHHVQMAFRLTAEPSHPQTKRFGTVLKQRVQVIQAEIQKYTSRRIRQSPLYSAQTPFKGDFHRLARRLCGRSVGLVLGGGGARGIAQVGVIKALEEAGIPIDVIGGTSIGSFIGALYARDADVVPMYGRAKKFAGRMGSIWRFALDLTYPTVSYTTGHEFNRGIFKTFGDSQIEDFWLEFYCNTTNISRSRAEYHSSGYTWRYVRASMSLAGLIPPICDEGSMLLDGGYIDNLTVDHMKGLGADVIFAVDVGSIDDNTPQVYGDSLSGFWAVVNRWNPFSSCPNPPTLSEIQARLAYVSSIENLERAKNTPGCLYMRPPIDPYGTLDFAKFDEIYQLGYAYGKNYLEKLKREGSLPLPEETEEKKKLQRTLAPRRASI |
| Enzyme Length | 1531 |
| Uniprot Accession Number | A2R350 |
| Absorption | |
| Active Site | ACT_SITE 1261; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1379; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 689..809; /note=cNMP 1; NP_BIND 849..969; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Erroneous gene model prediction (1); Motif (3); Nucleotide binding (2); Region (4); Topological domain (3); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1232..1237; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1259..1263; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1379..1381; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 169,000 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |