Detail Information for IndEnz0010001377
IED ID IndEnz0010001377
Enzyme Type ID esterase001377
Protein Name Lysophospholipase nte1
EC 3.1.1.5
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene Name nte1 An14g03050
Organism Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus niger Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Enzyme Sequence MATGDGIIAAPPSLESSSLDPLHVLPASSSTAARSLATSIPALTASFSVVSGFSSHLPPPPVTPPAPSTMVGWIGWIFSFIFQVIPSVLYWIVTFTTITLPTWLFTLFSMSLTFTMNFTTLLLIALAVVSTISWFIRYRFLNMYSRLPPEPQRKEPQLDLFPDVPDSDTKPGLANYLDEFLSAIKVFGYLERPVFHELTRTMQTRKLIAGETLLLEEEKGFCLVVDGLVQIFVKSMRDRKSGSDEELNRMAGESSDEDDHRPDGRQGYQLLTEVKNGASMSSLFSILSLFTEDVQLRYADSSASSASSIGPGLAIGPDSFPASPREMDDSPHVYQGDRLDPASHRNSTAEDLPAVPPLNLGESQVPPAHSARSESRKYSGKTRRKSVHPDIVARAMVDTTIAIIPASAFRRLTRVYPKATAHIVQVILTRLQRVTFATAHSYLGLNNEVLGIEKQMTKFTTYDLPNELRGSALDRLKDKFIKERDRLGQEEVTKGIALHNPYGGRRPRSSSFRRKEAALQAKMVASKRPVSMVAQDSALSDRENSGVSPGDLLSTIQLSRFGPRYEHLAPRLLSPLTEKEHSPLRSPSPMIPGRASPFHRKESLDEDALFRESILECIMKGIGLTGSTNDFLRKSSHPSGDVSPKLLSYDSRRQKAVFSNNAFGFIDPYEGSADGESESMMSMSVTSAGGTSPIVNLREELRNDIEIVYFPQGSVLVEQGERHPGLYYVVDGFLDVGIPVDDKEEDLVGSSRPAHEELFPMLRRTNTSSSRVSGSAAAANDPRRKKQSRRSLYLIKPGGIQGYVGAVASYRSYTDVVAKTDVYVGFLPRASLERIAERYPLALLTLAKRLTSLLPRLLLHIDFALEWVQVSAGQVIYHQGDESDAIYLALNGRLRSVHEGPNGKMTVVGEYGQGESVGELEVMTESTRPATLHAIRDTELAKFPRTLFNSLAQEHPGITIQVSKLIAQRMRDLVETPLAEKGGEPGVSGTVKTAKSTLNLRTVGILPVTAGVPVVEFGNRLLQALHQIGVTNGATSLNQAAILNHLGRHAFSKMGKLKLSQYLADLEEKYGMVLYIADTSVNSPWTQTCITQADCILLVGLAESTPSIGEYERFLLGMKTTARKELVLLHGERYCPPGLTRQWLKNRVWINGGHHHVQMAFRLTAEPSHPQTKRFGTVLKQRVQVIQAEIQKYTSRRIRQSPLYSAQTPFKGDFHRLARRLCGRSVGLVLGGGGARGIAQVGVIKALEEAGIPIDVIGGTSIGSFIGALYARDADVVPMYGRAKKFAGRMGSIWRFALDLTYPTVSYTTGHEFNRGIFKTFGDSQIEDFWLEFYCNTTNISRSRAEYHSSGYTWRYVRASMSLAGLIPPICDEGSMLLDGGYIDNLTVDHMKGLGADVIFAVDVGSIDDNTPQVYGDSLSGFWAVVNRWNPFSSCPNPPTLSEIQARLAYVSSIENLERAKNTPGCLYMRPPIDPYGTLDFAKFDEIYQLGYAYGKNYLEKLKREGSLPLPEETEEKKKLQRTLAPRRASI
Enzyme Length 1531
Uniprot Accession Number A2R350
Absorption
Active Site ACT_SITE 1261; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1379; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161
Activity Regulation ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5;
DNA Binding
EC Number 3.1.1.5
Enzyme Function FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 689..809; /note=cNMP 1; NP_BIND 849..969; /note=cNMP 2
Features Active site (2); Chain (1); Compositional bias (1); Domain (1); Erroneous gene model prediction (1); Motif (3); Nucleotide binding (2); Region (4); Topological domain (3); Transmembrane (2)
Keywords Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 1232..1237; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1259..1263; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1379..1381; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161
Gene Encoded By
Mass 169,000
Kinetics
Metal Binding
Rhea ID RHEA:15177
Cross Reference Brenda