| IED ID | IndEnz0010001378 |
| Enzyme Type ID | esterase001378 |
| Protein Name |
Palmitoleoyl-protein carboxylesterase NOTUM Smed-notum EC 3.1.1.98 |
| Gene Name | notum |
| Organism | Schmidtea mediterranea (Freshwater planarian flatworm) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Spiralia Lophotrochozoa Platyhelminthes Rhabditophora Seriata Tricladida Continenticola Geoplanoidea Dugesiidae Schmidtea Schmidtea mediterranea (Freshwater planarian flatworm) |
| Enzyme Sequence | MKSYLILNTLLLSLLKINGFSKSSWFSSKTSLIFDRINKLNDPQSSNSIHSRKYQYFQLKKFPNSTNVRCNDGSIPGYYTRPSTTNCSKKWLIFLEGGWYCFNNNTCESRRRTHYDLFSSEFWSSERQLGGILSNNERINPNFHDYNSVYIPYCSSDLWSGKQLEKTNGLYFHGSRILDTVVDDLTQNQHFKKVHEVAFVGSSAGGIGVLLNIDRLKRRLKKKLKRKVFIHGIVDSAWFLDYPAYRQSNCTHIYECPPENALRNGMKLWNPRIPRRCKKFQGRGREWKCFMGPVIYRHLKNPTFIIQSLFDDAQLQMSKVPILEGGSNKKFSYIQQLGGFAAQTLRQAKGVFAHSCVDHEILTKSNWAYVSVNNQRLHETLNYWQAYLEGEKKKIKKKVQKNPKLIKTGKSPCKNLRKPKFSGNIDQSKYQLIDSCHISQITSYKIQLPHNRTLSRCANAIPLIPLCNPTCSPLSHPISGLSMSFIDLLELYNVRINLIAKSLGISMEQLRKMNTQQQISLLYCSSR |
| Enzyme Length | 527 |
| Uniprot Accession Number | F8U830 |
| Absorption | |
| Active Site | ACT_SITE 203; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 311; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 359; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[Wnt protein]-O-(9Z)-hexadecenoyl-L-serine + H2O = (9Z)-hexadecenoate + [Wnt protein]-L-serine + H(+); Xref=Rhea:RHEA:45340, Rhea:RHEA-COMP:11170, Rhea:RHEA-COMP:11171, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:32372, ChEBI:CHEBI:85189; EC=3.1.1.98; Evidence={ECO:0000250|UniProtKB:Q6P988}; |
| DNA Binding | |
| EC Number | 3.1.1.98 |
| Enzyme Function | FUNCTION: Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway (PubMed:21566195). Acts by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (By similarity). Promotes head regeneration following amputation by inhibiting the Wnt signaling pathway. {ECO:0000250|UniProtKB:Q6P988, ECO:0000269|PubMed:21566195}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (5); Signal peptide (1) |
| Keywords | Glycoprotein;Hydrolase;Secreted;Serine esterase;Signal;Wnt signaling pathway |
| Interact With | |
| Induction | INDUCTION: Strongly up-regulated near anterior-facing wounds following head and tail amputation. {ECO:0000269|PubMed:21566195}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VUX3}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 60,852 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:45340 |
| Cross Reference Brenda | 3.1.1.98; |