| IED ID | IndEnz0010001379 |
| Enzyme Type ID | esterase001379 |
| Protein Name |
Palmitoleoyl-protein carboxylesterase notum1a EC 3.1.1.98 |
| Gene Name | notum1a |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MKRSLWVMQVLHWAVMLALVQCGALGARRFRGGRNPQPRRALPSAHYRDRGETTESFSLDFTAVEENMDNFMTQVKNLAQSLYPCSAQKLDYDMKLHFLENTSVTCNDGTPAGYYLKESKGSKRWLIFLEGGWYCFNKENCDSRYETMRRLMSSSKWPQTKTGTGMLSSLPEENPHWWNANMVFIPYCSSDVWSGASPKTDQNDYAFMGSLIIKEVVKDLLSKGLDNAKILLLAGSSAGGTGVLLNVDSVSELLEELGHTNIQVRGLSDSGWFLDNKQYRCTDCVDTINCAPTEVIKRGIKYWGGVVPERCRQAYEGKEWNCFFGYKVYPTIKRPVFIVQWLFDEAQLTVDNIHLTGQPVQEGQWRYIQNLGTELRNTLKDVPAMFAPACLSHEFITRNYWTDVQVKGTSLPRALHCWDRSLQDTSRNNKSPPKGCPVHLIDSCPWPHCNPTCPTIRDQSTGQEMNVIQFLMHMGFDVQKMAHQQGMDPSKLLGMLSSGS |
| Enzyme Length | 500 |
| Uniprot Accession Number | C5H5C4 |
| Absorption | |
| Active Site | ACT_SITE 237; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 344; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 393; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[Wnt protein]-O-(9Z)-hexadecenoyl-L-serine + H2O = (9Z)-hexadecenoate + [Wnt protein]-L-serine + H(+); Xref=Rhea:RHEA:45340, Rhea:RHEA-COMP:11170, Rhea:RHEA-COMP:11171, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:32372, ChEBI:CHEBI:85189; EC=3.1.1.98; Evidence={ECO:0000250|UniProtKB:Q6P988}; |
| DNA Binding | |
| EC Number | 3.1.1.98 |
| Enzyme Function | FUNCTION: Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway (PubMed:22669824). Acts by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (By similarity). {ECO:0000250|UniProtKB:Q6P988, ECO:0000269|PubMed:22669824}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (1); Signal peptide (1) |
| Keywords | Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal;Wnt signaling pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VUX3}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 23293289; 23922899; 28837622; |
| Motif | |
| Gene Encoded By | |
| Mass | 56,715 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:45340 |
| Cross Reference Brenda | 3.1.1.98; |