| IED ID | IndEnz0010001393 |
| Enzyme Type ID | esterase001393 |
| Protein Name |
Palmitoleoyl-protein carboxylesterase notum1 EC 3.1.1.98 |
| Gene Name | notum1 |
| Organism | Xenopus laevis (African clawed frog) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Xenopus Xenopus laevis (African clawed frog) |
| Enzyme Sequence | MAGALCVTLLLLLSTNTVSGRKTWRRRGQQIVPSGRERSEGGDESFPLDFTAVEGNMDNFMAQIKSLAQSLYPCSAQRLDDEMKLHILHNKSVTCNDGSSAGYYLKESKGSRRWLVFLEGGWYCISHENCDLRYDTMRRLMSSKAWPPAKTASGILSTQPEENPHWWNANMVFIPYCSSDVWSGASPKTEKSGYAFMGSLIIQEVVKELLGKGLDAAKVLLLAGSSAGGTGVLLNVDLVADLLEELGYPGIQVRGLSDSGWFLDNKQYRRTDCTDIITCAPTEAIQRGIRYWSSMVPERCKQQFKEGEEWNCFFGYKIYPTLRSPVFVVQWLFDEAQLTVDNVHLSGQPVQESQWLYIQNLGRELRNTLKDVGASFAPACLAHEVITRSHWTEIQVRGTSLPRALHCWDRRLQETNKNSKVPLKGCPFHLMDSCPWPQCNPTCPSIRDHFTGQEMSVVQFLMHLGFDVQKMASQQGMEPGKLLGVLSS |
| Enzyme Length | 488 |
| Uniprot Accession Number | A0A0D3QS99 |
| Absorption | |
| Active Site | ACT_SITE 226; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 334; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 383; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[Wnt protein]-O-(9Z)-hexadecenoyl-L-serine + H2O = (9Z)-hexadecenoate + [Wnt protein]-L-serine + H(+); Xref=Rhea:RHEA:45340, Rhea:RHEA-COMP:11170, Rhea:RHEA-COMP:11171, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:32372, ChEBI:CHEBI:85189; EC=3.1.1.98; Evidence={ECO:0000250|UniProtKB:Q6P988}; |
| DNA Binding | |
| EC Number | 3.1.1.98 |
| Enzyme Function | FUNCTION: Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors. Functions in the prospective ectoderm and is required for neural induction. {ECO:0000305|PubMed:25771893}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (1); Signal peptide (1) |
| Keywords | Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal;Wnt signaling pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VUX3}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 54,784 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:45340 |
| Cross Reference Brenda | 3.1.1.98; |