IED ID | IndEnz0010001399 |
Enzyme Type ID | esterase001399 |
Protein Name |
Iron III enterobactin esterase EC 3.1.1.108 Fe-Ent esterase |
Gene Name | fes c0671 |
Organism | Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) |
Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) |
Enzyme Sequence | MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYSTIKRVWVYITGVTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIFSAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQAPETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSLTHRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTVVAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLNTGEVSAEGLRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLWQPLFHDRS |
Enzyme Length | 400 |
Uniprot Accession Number | A0A0H2V760 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Fe(III)-enterobactin + H(+) + 3 H2O = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine] + 2 N-(2,3-dihydroxybenzoyl)-L-serine; Xref=Rhea:RHEA:30111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28199, ChEBI:CHEBI:58154, ChEBI:CHEBI:143010; EC=3.1.1.108; Evidence={ECO:0000269|PubMed:16076215};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30112; Evidence={ECO:0000269|PubMed:16076215}; CATALYTIC ACTIVITY: Reaction=Fe(III)-enterobactin + H2O = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]3 + H(+); Xref=Rhea:RHEA:59256, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28199, ChEBI:CHEBI:143011; Evidence={ECO:0000250|UniProtKB:P13039};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59257; Evidence={ECO:0000250|UniProtKB:P13039}; CATALYTIC ACTIVITY: Reaction=Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]3 + H(+) + H2O = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]2 + N-(2,3-dihydroxybenzoyl)-L-serine; Xref=Rhea:RHEA:59260, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58154, ChEBI:CHEBI:143011, ChEBI:CHEBI:143012; Evidence={ECO:0000250|UniProtKB:P13039};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59261; Evidence={ECO:0000250|UniProtKB:P13039}; CATALYTIC ACTIVITY: Reaction=Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]2 + H(+) + H2O = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine] + N-(2,3-dihydroxybenzoyl)-L-serine; Xref=Rhea:RHEA:59264, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58154, ChEBI:CHEBI:143010, ChEBI:CHEBI:143012; Evidence={ECO:0000250|UniProtKB:P13039};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59265; Evidence={ECO:0000250|UniProtKB:P13039}; |
DNA Binding | |
EC Number | 3.1.1.108 |
Enzyme Function | FUNCTION: Catalyzes the hydrolysis of ferric enterobactin (Fe-Ent). Is responsible for the release of iron from ferric enterobactin. Also catalyzes the hydrolysis of iron-free enterobactin (Ent). Hydrolyzes ferric monoglucosyl-C-Ent (Fe-MGE) poorly and does not hydrolyze ferric diglucosyl-C-Ent (Fe-DGE) or ferric triglucosyl-C-Ent (Fe-TGE) at all. Also hydrolyzes apo MGE, but catalyzes the hydrolysis of apo DGE very poorly, and does not process apo TGE at all. The catalytic efficiency for processing Fe-Ent is much higher than that for apo Ent, suggesting that Fe-Ent is the physiological substrate. {ECO:0000269|PubMed:16076215}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Chain (1) |
Keywords | Cytoplasm;Hydrolase |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P13039}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 45,641 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=100 uM for MGE {ECO:0000269|PubMed:16076215}; KM=0.29 uM for Fe-MGE {ECO:0000269|PubMed:16076215}; Note=kcat is 9 min(-1) with Fe-Ent as substrate. kcat is 850 min(-1) with MGE as substrate. kcat is 1.4 min(-1) with Fe-MGE as substrate. {ECO:0000269|PubMed:16076215}; |
Metal Binding | |
Rhea ID | RHEA:30111; RHEA:30112; RHEA:59256; RHEA:59257; RHEA:59260; RHEA:59261; RHEA:59264; RHEA:59265 |
Cross Reference Brenda |