Detail Information for IndEnz0010001405
IED ID IndEnz0010001405
Enzyme Type ID esterase001405
Protein Name Peptidoglycan deacetylase
PG deacetylase
EC 3.5.1.-
Acetylxylan esterase
EC 3.1.1.72
Gene Name pgdA HP_0310 C694_01565
Organism Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria delta/epsilon subdivisions Epsilonproteobacteria Campylobacterales Helicobacteraceae Helicobacter Helicobacter pylori (Campylobacter pylori) Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Enzyme Sequence MAKEILVAYGVDIDAVAGWLGSYGGEDSPDDISRGLFAGEVGIPRLLKLFKKYHLPATWFSPGHSIETFSEQMKMIVDAGHEVGAHGYSHENPIAMTAKQEEDVLLKSVELIKDLTGKAPTGYVAPWWEFSNITNELLLKHGFKYDHSLMHNDFTPYYVRVGDSWSKIDYSLEAKDWMKPLIRGVETDLVEIPANWYLDDLPPMMFIKKSPNSFGFVSPHDIGQMWIDQFDWVYREMDYAVFSMTIHPDVSARPQVLLMHEKIIEHINKHEGVRWVTFNEIADDFLKRNPRKK
Enzyme Length 293
Uniprot Accession Number O25080
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Deacetylation of xylans and xylo-oligosaccharides.; EC=3.1.1.72; Evidence={ECO:0000269|PubMed:23825549};
DNA Binding
EC Number 3.5.1.-; 3.1.1.72
Enzyme Function FUNCTION: Catalyzes the N-deacetylation of peptidoglycan (PG), an important mechanism that appears to confer lysozyme resistance and to mitigate host immune detection; this likely contributes to pathogen persistence in the host. The exact nature of the residue in PG that is deacetylated has not been determined. Is also able to catalyze the deacetylation of acetylated xylan, and, to a lesser extent, that of chitin and chitosan. Therefore, this enzyme might play a role during infection, considering that xylan-containing carbohydrate structures are among those commonly consumed by humans. {ECO:0000269|PubMed:19147492, ECO:0000269|PubMed:20805339, ECO:0000269|PubMed:23221800, ECO:0000269|PubMed:23825549}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius with acetylated xylan as substrate. Retains about 80% of the activity at 30-55 degrees Celsius. {ECO:0000269|PubMed:23825549};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0 with acetylated xylan as substrate. Is active at acidic pH ranging from 5.0 to 6.0. {ECO:0000269|PubMed:23825549};
Pathway
nucleotide Binding
Features Chain (1); Domain (1); Initiator methionine (1); Metal binding (3)
Keywords Carbohydrate metabolism;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Metal-binding;Polysaccharide degradation;Reference proteome;Stress response;Xylan degradation;Zinc
Interact With
Induction INDUCTION: Its expression is markedly up-regulated upon cell exposure to oxidative stress. Is also significantly induced (3.5-fold) when H.pylori cells are in contact with macrophages. {ECO:0000269|PubMed:19147492, ECO:0000269|PubMed:20805339}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 33,677
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.85 mM for chitin {ECO:0000269|PubMed:23825549}; KM=0.85 mM for chitosan {ECO:0000269|PubMed:23825549}; KM=0.42 mM for acetylated xylan {ECO:0000269|PubMed:23825549}; KM=0.18 mM for pNP-acetate {ECO:0000269|PubMed:23825549}; Note=kcat is 775 min(-1), 968 min(-1), 2549 min(-1) and 1029 min(-1) with chitin, chitosan, acetylated xylan, and pNP-acetate as substrate, respectively.;
Metal Binding METAL 14; /note=Zinc; /evidence=ECO:0000250; METAL 86; /note=Zinc; via tele nitrogen; /evidence=ECO:0000250; METAL 90; /note=Zinc; via tele nitrogen; /evidence=ECO:0000250
Rhea ID
Cross Reference Brenda 3.5.1.104;