Detail Information for IndEnz0010001444
IED ID IndEnz0010001444
Enzyme Type ID esterase001444
Protein Name Senescence-associated carboxylesterase 101
EC 3.1.1.1
Gene Name SAG101 At5g14930 F2G14.50
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MESSSSLKGSALGKLVVTSGLLHSSWSKILEIHNPPYSNHDPGLQVSKKKKDSGLEFQIHREEKFTLVVFSAPPICRSSSSDSTLLHVKDKENPFPFLCSENNPSFSLHTPAFNLFTSASTSLTYLKSELLQTLKSEKPVIITGAALGGSVASLYTLWLLETIEPTLKRPLCITFGSPLIGDASLQQILENSVRNSCFLHVVSAQTRIKMDFFKPFGTFLICFDSGCVCIEDHVAVTELLNGVHDSGLVDYSQVLNRLDQSMLSLADSRLIPEDVIKGIEKRAEMKNLRFDMMFKKLNDMKISMAYIEWYKKKCKEVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASILKRRFLFSGNNYRRMIEPLDIAEYYLEGRKEYRTTGRSHHYVMLEKWFGMESILIEKERCKKRDLSDLLTFDSCFWAEVEDSLIVINQLNTTVGMRDDVREVLTRKLVEFEGYVWEIITKREVSPEIFLEESSFMKWWKEYKKIKGFNSSYLTEFMNTRKYESYGKSQ
Enzyme Length 537
Uniprot Accession Number Q4F883
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000269|PubMed:11971136};
DNA Binding
EC Number 3.1.1.1
Enzyme Function FUNCTION: Acyl hydrolase that triggers the leaf senescence onset. Can use triolein as substrate to produce oleic acids.; FUNCTION: Involved in the EDS1-dependent intrinsic and indispensable resistance signaling pathway; together with PAD4, required for programmed cell death triggered by RPS4 in response to avirulent pathogens (e.g. P.syringae pv. tomato strain DC3000 and H.parasitica isolates CALA2 and EMWA1) and in restricting the growth of virulent pathogens (e.g. H.parasitica isolates NOCO2 and P.syringae pv. tomato strain DC3000 avrRps4). Contributes in reinforcing the immune response around hypersensitive response foci (PubMed:21434927). Regulates the nuclear localization of EDS1. Essential for the RPP8/HRT-mediated resistance to the turnip crinkle virus (TCV). Involved in the post-invasion resistance to P.pachyrhizi in the mesophyll. {ECO:0000269|PubMed:21434927, ECO:0000269|PubMed:22072959}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Alternative sequence (2); Beta strand (11); Chain (1); Erroneous gene model prediction (1); Helix (27); Mutagenesis (4); Sequence conflict (1); Signal peptide (1); Transmembrane (1); Turn (3)
Keywords 3D-structure;Alternative splicing;Cytoplasm;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Nucleus;Plant defense;Reference proteome;Signal;Transmembrane;Transmembrane helix
Interact With
Induction INDUCTION: Specifically induced during senescence via a complex epigenetic processe involving histone methylation. {ECO:0000269|PubMed:19143996}.
Subcellular Location SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. Nucleus {ECO:0000269|PubMed:22072959}. Cytoplasm {ECO:0000269|PubMed:22072959}. Note=Can move to the cytoplasm when in complex with PAD4 and EDS1.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..27; /evidence=ECO:0000255
Structure 3D X-ray crystallography (1)
Cross Reference PDB 4NFU;
Mapped Pubmed ID 15282545; 15604721; 15990873; 16299169; 16514542; 16603661; 16913859; 17142483; 17227549; 17885809; 18650403; 18705666; 19578402; 23505340; 25681297; 25740259; 25915452; 26149542; 26340231; 26505534; 28351989; 28475615; 29253890; 29931201; 31311833; 31550533; 32396762; 34099661;
Motif
Gene Encoded By
Mass 62,066
Kinetics
Metal Binding
Rhea ID RHEA:21164
Cross Reference Brenda