Detail Information for IndEnz0010001461
IED ID IndEnz0010001461
Enzyme Type ID esterase001461
Protein Name Probable feruloyl esterase B-2
EC 3.1.1.73
Ferulic acid esterase B-2
FAEB-2
Gene Name faeB-2 ATEG_06663
Organism Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Enzyme Sequence MAPIHYLLPIITLGSAALARQDAFEAKCHSFANKIHLPNVHVNFASYVPGGTNLTLADNPSSCGATSQSVSADVCRVAMAVATSNSSEITLEAWFPRNYTGRFLSTGNGGLSGCIQYYDMAYTTGFGFATVGANNGHNGTSGEPFYHHPEVLEDFAYRSIHTGVVIGKKLTKMFYEEGFNKSYYLGCSTGGRQGFKSVQKYPNDFDGVVAGAPAFNFANLISWSAHFYPITGPPGSDTYLSPAMWKVAHDEIIRQCDQIDGAKDGIIEDPSLCNPIMETIICKPGASSDNCLSAAQAKTVREVLYPLYGVNGTLLYPRMQPGSEVLAAPIMYNGQPFAYSTDWYRYVVYNDPNWNGTTFDVQDAAAALAQNPYNIQTWDADLTPFRKSGGKVLTYHGLQDQLISSENSKLYYARVAETMGMPPEELDEFYRFFQISGMGHCGGGDGAYGIGNGLATYSGKDPENNVLMAMVQWVEKGIAPETVRGAKFANGPGSTVEYSRKHCRYPRRNVFKGPGNYTDENAWECVV
Enzyme Length 527
Uniprot Accession Number Q0CI21
Absorption
Active Site ACT_SITE 188; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q2UP89; ACT_SITE 400; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q2UP89; ACT_SITE 440; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q2UP89
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide.; EC=3.1.1.73; Evidence={ECO:0000250|UniProtKB:Q8WZI8};
DNA Binding
EC Number 3.1.1.73
Enzyme Function FUNCTION: Involved in degradation of plant cell walls. Hydrolyzes the feruloyl-arabinose ester bond in arabinoxylans as well as the feruloyl-galactose and feruloyl-arabinose ester bonds in pectin. {ECO:0000250|UniProtKB:Q8WZI8}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (6); Erroneous gene model prediction (1); Glycosylation (8); Metal binding (5); Signal peptide (1)
Keywords Calcium;Carbohydrate metabolism;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Polysaccharide degradation;Reference proteome;Secreted;Serine esterase;Signal;Xylan degradation
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 57,504
Kinetics
Metal Binding METAL 257; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 260; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 262; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 264; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 266; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q2UP89
Rhea ID
Cross Reference Brenda