| IED ID | IndEnz0010001465 |
| Enzyme Type ID | esterase001465 |
| Protein Name |
Peroxisomal membrane protein LPX1 EC 3.1.1.- Lipase of peroxisomes protein 1 |
| Gene Name | LPX1 YOR084W YOR3120W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MEQNRFKKETKTCSASWPRAPQSTLCATDRLELTYDVYTSAERQRRSRTATRLNLVFLHGSGMSKVVWEYYLPRLVAADAEGNYAIDKVLLIDQVNHGDSAVRNRGRLGTNFNWIDGARDVLKIATCELGSIDSHPALNVVIGHSMGGFQALACDVLQPNLFHLLILIEPVVITRKAIGAGRPGLPPDSPQIPENLYNSLRLKTCDHFANESEYVKYMRNGSFFTNAHSQILQNIIDFERTKASGDDEDGGPVRTKMEQAQNLLCYMNMQTFAPFLISNVKFVRKRTIHIVGARSNWCPPQNQLFLQKTLQNYHLDVIPGGSHLVNVEAPDLVIERINHHIHEFVLTSPLQSSHIPQLTLEERAVMFDRAFDSFKNEALVKTTKQKL |
| Enzyme Length | 387 |
| Uniprot Accession Number | Q12405 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Has acyl esterase, lipase and phospholipase A activity. {ECO:0000269|PubMed:18199283}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (11); Chain (1); Helix (17); Region (1); Turn (4) |
| Keywords | 3D-structure;Hydrolase;Peroxisome;Reference proteome |
| Interact With | |
| Induction | INDUCTION: By oleic acid. Transcriptionally up-regulated by YRM1 along with genes involved in multidrug resistance. Expression is also dependent on RSF1 and RSF2 for transcriptional induction during growth on glycerol-based medium. {ECO:0000269|PubMed:12135984, ECO:0000269|PubMed:14512416, ECO:0000269|PubMed:18199283, ECO:0000269|PubMed:19235764}. |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000269|PubMed:12135984, ECO:0000269|PubMed:18199283}. Note=Peroxisomal import is dependent on the peroxisomal targeting signal type 1 (PTS1) receptor PEX5 and on self-interaction. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 2Y6U; 2Y6V; |
| Mapped Pubmed ID | 10688190; 14690591; 18621837; 19436716; 19536198; 19684113; 20124343; 21478430; 21741480; 21912624; 23275493; 23383298; 23518202; 24187129; 27693354; |
| Motif | |
| Gene Encoded By | |
| Mass | 43,727 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=6.3 uM for p-nitrophenyl butyrate (PNB) {ECO:0000269|PubMed:18199283}; Vmax=5.6 pmol/h/ug enzyme toward 1,2-dioleoyl-3-(pyren-1-yl)decanoyl-rac-glycerol (DPG) {ECO:0000269|PubMed:18199283}; Vmax=7.9 pmol/h/ug enzyme toward 1,2-bis-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-sindacene-3-undecanoyl)-sn-glycero-3-phosphocholine (BPC) {ECO:0000269|PubMed:18199283}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |