| IED ID | IndEnz0010001485 |
| Enzyme Type ID | esterase001485 |
| Protein Name |
Gluconolactonase EC 3.1.1.17 D-glucono-delta-lactone lactonohydrolase Cleaved into: Gluconolactonase; Gluconolactonase minor isoform |
| Gene Name | gnl ZMO1649 |
| Organism | Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Alphaproteobacteria Sphingomonadales Zymomonadaceae Zymomonas Zymomonas mobilis Zymomonas mobilis subsp. mobilis Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) |
| Enzyme Sequence | MTTGRMSRRECLSAAVMVPIAAMTATATITGSAQAAKNNMNGSTIGKITKFSPRLDAILDVSTPIEVIASDIQWSEGPVWVKNGNFLLFSDPPANIMRKWTPDAGVSIFLKPSGHAEPIPAGQFREPGSNGMKVGPDGKIWVADSGTRAIMKVDPVTRQRSVVVDNYKGKRFNSPNDLFFSKSGAVYFTDPPYGLTNLDESDIKEMNYNGVFRLSPDGRLDLIEAGLSRPNGLALSPDETKLYVSNSDRASPNIWVYSLDSNGLPTSRTLLRNFRKEYFDQGLAGLPDGMNIDKQGNLFASAPGGIYIFAPDGECLGLISGNPGQPLSNCCFGEKGQTLFISASHNVVRVRTKTFG |
| Enzyme Length | 356 |
| Uniprot Accession Number | Q01578 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=D-glucono-1,5-lactone + H2O = D-gluconate + H(+); Xref=Rhea:RHEA:10440, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16217, ChEBI:CHEBI:18391; EC=3.1.1.17; Evidence={ECO:0000305|PubMed:1482681}; |
| DNA Binding | |
| EC Number | 3.1.1.17 |
| Enzyme Function | FUNCTION: Hydrolyzes the gluconolactone formed by glucose-fructose oxidoreductase, and that formed in aerobic conditions by the glucose dehydrogenase present. {ECO:0000305|PubMed:1482681}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Carbohydrate acid metabolism; D-gluconate biosynthesis; D-gluconate from D-glucono-1,5-lactone: step 1/1. |
| nucleotide Binding | |
| Features | Chain (2); Sequence conflict (3); Signal peptide (1) |
| Keywords | Direct protein sequencing;Hydrolase;Periplasm;Reference proteome;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Periplasm {ECO:0000305|PubMed:1482681}. |
| Modified Residue | |
| Post Translational Modification | PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. {ECO:0000255|PROSITE-ProRule:PRU00648, ECO:0000269|PubMed:1482681}. |
| Signal Peptide | SIGNAL 1..35; /note="Tat-type signal"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00648, ECO:0000269|PubMed:1482681" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,453 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:10440 |
| Cross Reference Brenda | 3.1.1.17; |