Detail Information for IndEnz0010001495
IED ID IndEnz0010001495
Enzyme Type ID esterase001495
Protein Name Putative methylesterase 14, chloroplastic
AtMES14
EC 3.1.1.-
Gene Name MES14 At1g33990 F12G12.19 F12G12.220 T15K4.4
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MGNKIISMMKKDSKDGGGGGSKSKRMNRSQRKLLADEEMLHRRALSMAIHQAQLSQRFDGSMSRRVGSTSTRKRTLSDPFSNGKQVPDFSESLIVKKFVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIAQIP
Enzyme Length 348
Uniprot Accession Number Q9FVW3
Absorption
Active Site ACT_SITE 172; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 299; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 327; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Putative methylesterase.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Alternative sequence (2); Chain (1); Erroneous gene model prediction (1); Modified residue (1); Region (2); Sequence conflict (1); Transit peptide (1)
Keywords Alternative splicing;Chloroplast;Hydrolase;Phosphoprotein;Plastid;Reference proteome;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
Modified Residue MOD_RES 77; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:19376835
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 17317660; 17644812; 18643994; 18650403; 20736450;
Motif
Gene Encoded By
Mass 38,855
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda