| IED ID | IndEnz0010001496 |
| Enzyme Type ID | esterase001496 |
| Protein Name |
Lysophospholipase nte1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | nte1 AO090012000173 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MATDGGPLAASSASLDSSLSPLHASPSPSSTYTASSLALSAPAIAASFSVASTFSNSLIPPPPLPPPTPSTMAGWFGWVFSFFFQVIPSVLYWVITFATITLPTWLFTLFSMSLTFTMNFTTLLLIALAIVSTISWFIRYRFLNMYSRLPSEPQRKEPHLDLFPDVQEGDSKPGLANYLDEFLSAIKVFGYLERPVFHELTRTMQTRKLIAGETLMLEEEKGFCLVVDGLVQIFVKSMRDGKPNVDEGSNHMGAESSDEDDHRVDGKQGYQLLTEVKNGASMSSLFSILSLFTEDIQLRASEGSSSSASSVGPSTNARVSDSFPASPHGLEDSPRSNFVRDHGDSVAHISNSNGELLPSVPPLNLGESHAMPIQEPYPKPRSQPGKRRRKSVHPDIVARAMVDTTIAIIPASAFRRLTRVYPRATAHIVQVILTRLQRVTFATAHSYLGLNNDVLGIEKQMTKFTTQDLPNEMRGAALDRLKDKFIKERDRLGPEEIIKGIALHNPFAGRRRRSSSFLRKEAVLHAKMAAQSKRPVSMASPEDISGDRESAGPSPGDLLSTIQLSRFGPRYEHLAPKLLSPLTDKENPPFMAPVMHSSPFHRKKDAVDEDALFRESILDCIMNGIGLTSSTRDVLRKSSHTSGDISPKLLSYDSRRQKAVFTNNAFGFIDPYDSSADGETESMMSMSMTSAGGTSPIVNLREELRNDIEIVYFPQGSVLVEQGERHPGLYYVIDGFLDVGIPVSDKGEDLVGGSRPVYGQPPEEFFPTLKRTTTSSSRVSGVTSATNDTKRKRQSRKSLYLIKPGGIQGYVGSVASYRSYTDVVAKTDVYVGFLPRSSLERIAERYPIALLTLAKRLTSLLPRLLLHIDFALEWVQVSAGQVIYHQGDESDAIYLVLNGRLRSVLEGTDGKITVVGEYGQGESVGELEVMTESTRPATLHAIRDTELAKFPRSLFNSLAQEHPGITIQVSKLIAQRMRDLVELPMPEKGGEHANVGSVKTAASVVNLRTVGILPVTAGVPVVEFGHRLQNALHQIGVTNGVTSLNQAAILNHLGRHAFSKMGKLKLSQYLADLEEKYGMVLYIADTNVNSPWTQTCITQADCILLVGLAESSPSIGEYERFLLGMKTTARKELVLLHSERYCPPGLTRQWLKNRMWINGGHHHIQMGFRLTPEPSHPQAKRLGAVLKQRVQVIQAEIQKYTSRRIRQTPLYSAQTPFKGDFHRLARRLCGRSVGLVLGGGGARGIAQVGVIKALEEAGIPIDIIGGTSIGSFIGALYARDADVVPMYGRAKKFAGRMASMWRFMLDLTYPTTSYTTGHEFNRGIFKTFGDSQIEDFWLEYYCNTTNISKSRPEFHSSGYTWRYVRASMSLAGLIPPICDEGSMLLDGGYIDNLTVTHMKGLGADVIFAVDVGSIDDNTPQGYGDSLSGFWTVLNRWNPFSACPNPPTLSEIQARLAYVSSIDNLERAKNTPGCLYMRPPIDPYGTLEFGKFDEIYQVGYAYGKQYLEKLRSEGSLPLPEETEEKKKLQRTLAPRRASI |
| Enzyme Length | 1538 |
| Uniprot Accession Number | Q2UDH2 |
| Absorption | |
| Active Site | ACT_SITE 1268; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1386; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 692..811; /note=cNMP 1; NP_BIND 856..976; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Erroneous gene model prediction (1); Motif (3); Nucleotide binding (2); Region (4); Topological domain (3); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1239..1244; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1266..1270; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1386..1388; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 169,603 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |