| IED ID | IndEnz0010001511 |
| Enzyme Type ID | esterase001511 |
| Protein Name |
Tannase EC 3.1.1.20 Tannin acylhydrolase Fragment |
| Gene Name | |
| Organism | Bacillus subtilis |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis |
| Enzyme Sequence | VQPPHSHGDNFYIWT |
| Enzyme Length | 15 |
| Uniprot Accession Number | C0HJL9 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Activity is enhanced by the addition of 1 mM Mg(2+) (more than 100%) and by the addition of 1% glycerol (22%). Activity is reduced by the addition of 1 mM of various metal ions, including Hg(2+) (98%), Fe(3+) (95%), Fe(2+) (94%), Ba(2+) (92%), Ca(2+) (76%), Co(2+) (70%), Zn(2+) (44%), Ag(2+) (14%) and K(+) (8%). Inhibited by the addition of 1 mM DMSO (75%), Triton X-100 (42%), sodium azide (40%), beta-mercaptoethanol (34%), Tween 80 (22%), Tween 60 (20%), EDTA (18%) and phenylmethanesulfonyl fluoride (PMSF) (7%) but not affected by the addition of SDS. Also inhibited by the addition of 1% of acetic acid (73%), acetone (67%) and butanol (40%) but not affected by the addition of isopropanol, methanol and ethanol. {ECO:0000269|Ref.2}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=digallate + H2O = 2 3,4,5-trihydroxybenzoate + H(+); Xref=Rhea:RHEA:16365, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16918, ChEBI:CHEBI:17866; EC=3.1.1.20; Evidence={ECO:0000269|Ref.2}; |
| DNA Binding | |
| EC Number | 3.1.1.20 |
| Enzyme Function | FUNCTION: Hydrolyzes ester bonds of tannic acid to produce gallic acid and glucose. Exhibits no cytotoxicity in vitro in Vero cells or in vivo in a rat model. {ECO:0000269|Ref.2}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 40 degrees Celsius. Retains 14% of its maximal activity after heating 1 hour at 70 degrees Celsius. {ECO:0000269|Ref.2}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0. Active from pH 3.0 to 7.0. {ECO:0000269|Ref.2}; |
| Pathway | |
| nucleotide Binding | |
| Features | Non-terminal residue (1); Peptide (1) |
| Keywords | Direct protein sequencing;Hydrolase;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,798 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.445 mM for tannic acid at 40 degrees Celsius and pH 5.0 {ECO:0000269|Ref.2}; Vmax=125.8 mM/min/mg enzyme with tannic acid as substrate {ECO:0000269|Ref.2}; Note=Exhibits salt tolerance as it retains 67% of activity after 24 h exposure to 4 M NaCl. {ECO:0000269|Ref.2}; |
| Metal Binding | |
| Rhea ID | RHEA:16365 |
| Cross Reference Brenda |